Beta sheet tapes ribbons in tissue engineering

Patent metadata

Field	Value
Publication number	US-9951103-B2
Application number	US-201715460950-A
Country	US
Kind code	B2
Filing date	Mar 16, 2017
Priority date	Jul 15, 2002
Publication date	Apr 24, 2018
Grant date	Apr 24, 2018

Abstract

There is described a material comprising tapes, ribbons, fibrils or fibers characterized in that each of the ribbons, fibrils or fibers have an antiparallel arrangement of peptides in a β-sheet tape-like substructure.

First claim

The invention claimed is:

1. A self assembling polypeptide (SAP) that forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, and wherein the polypeptide consists of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), wherein an N-terminus of the peptide is blocked with CH 3 CO- and a C-terminus of the peptide is blocked with NH 2 —.

2. A dental product comprising the self-assembling polypeptide of claim 1 .

3. A dental repair or reconstruction or preventative product comprising the self-assembling polypeptide of claim 1 .

4. A tissue engineering scaffold comprising the self-assembling polypeptide of claim 1 .

5. The tissue engineering scaffold according to claim 4 , wherein the scaffold is seeded with cells.

6. A bone repair or reconstruction product comprising the self-assembling polypeptide of claim 1 .

7. The bone repair or reconstruction product according to claim 6 , wherein the product is seeded with cells.

8. A soft tissue or reconstruction product comprising the self-assembling polypeptide of claim 1 .

9. A skin treatment comprising the self-assembling polypeptide of claim 1 .

10. A hair care product comprising the self-assembling polypeptide of claim 1 .

11. A bioresponsive and biocompatible surface comprising the self-assembling polypeptide of claim 1 .

12. The self-assembling polypeptide of claim 1 , comprising continuous filaments, yarns, staple, floc, cord, or fabric.

13. The self-assembling polypeptide of claim 1 , further comprising a polymer.

14. The self-assembling polypeptide of claim 1 , wherein the physiological pH is about 7.5.

15. The self-assembling polypeptide of claim 1 , wherein the physiological salt concentration is about 145 mM NaCl.

16. A composition, comprising:
ribbons, fibrils or fibres, wherein each of the ribbons, fibrils or fibres has an antiparallel arrangement of peptides in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, wherein the composition comprises
a peptide consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12); and
a physiological concentration of salt.

17. The composition of claim 16 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.

18. A dental composition comprising the fibrils of claim 16 .

19. A dental repair or reconstruction or preventative composition comprising the fibrils of claim 16 .

20. The composition of claim 16 , wherein the composition is in the form of a tissue engineering scaffold.

21. The composition according to claim 20 , comprising the scaffold seeded with cells.

22. A bone repair or reconstruction composition comprising the fibrils of claim 16 .

23. A soft tissue or reconstruction composition comprising the fibrils of claim 16 .

24. The composition according to claim 22 that is seeded with cells.

25. A skin treatment comprising the composition of fibrils of claim 16 .

26. A hair care product comprising the composition of fibrils of claim 16 .

27. A part of a bioresponsive and biocompatible surface comprising the composition of fibrils of claim 16 .

28. The composition of claim 16 , wherein the composition comprises continuous filament yarns, staple, floc, cord, or fabric.

29. The composition of claim 16 , further comprising a polymer.

30. The composition of claim 16 , wherein the physiological pH is about 7.5.

31. The composition of claim 16 , wherein the physiological salt concentration is about 145 mM NaCl.

32. A method of forming a tape, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a tape.

33. A method of forming a gel, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a gel.

34. A method of forming a tape, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a tape.

35. A method of forming a gel, comprising contacting the composition of claim 16 with an ionic aqueous medium, thereby forming a gel.

36. A composition comprising:
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
an aqueous solution at a physiological pH and comprising a physiological concentration of salt,
wherein the peptide forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at the physiological pH and at the physiological concentration of salt.

37. The composition of claim 36 , wherein the physiological pH is pH 7 to pH 8, and the physiological concentration of salt is 120 mM to 160 mM NaCl.

38. The composition of claim 36 , wherein the physiological pH is pH 7.5 and the physiological concentration of salt is about 145 mM NaCl.

39. A composition comprising:
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
a cotton or wool fiber.

40. A tissue engineering scaffold, comprising;
a self assembling polypeptide (SAP) consisting of P 11-20 (QQRQEQEQEQQ, SEQ ID NO: 12), and
ligamentum cells, tenocytes, chondrocytes and/or chondrocyte progenitor cells.

Assignees

• Univ Leeds Innovations Ltd

Inventors

• Boden Neville
• Aggeli Amalia
• Ingham Eileen
• Kirkham Jennifer

Classifications

• A61K8/64

  Proteins; Peptides; Derivatives or degradation products thereof · CPC title

• A61L2430/34

  for soft tissue reconstruction · CPC title

• A61L27/22

  Polypeptides or derivatives thereof {, e.g. degradation products} · CPC title

• C09K8/62

  Compositions for forming crevices or fractures · CPC title

• A61Q19/00

  Preparations for care of the skin · CPC title