Beta sheet tapes ribbons in tissue engineering

Patent metadata

Field	Value
Publication number	US-2016340392-A1
Application number	US-201615234036-A
Country	US
Kind code	A1
Filing date	Aug 11, 2016
Priority date	Jul 15, 2002
Publication date	Nov 24, 2016
Grant date	—

Abstract

There is described a material comprising tapes, ribbons, fibrils or fibres characterized in that each of the ribbons, fibrils or fibres have an antiparallel arrangement of peptides in a β-sheet tape-like substructure wherein the material comprises a pair of self assembling complementary polypeptides.

First claim

1 . A pair of self assembling complementary polypeptides (SACPs) that form tapes, ribbons, fibrils or fibres in a β-sheet tape-like substructure, when a first monomeric peptide is contacted or mixed with its complementary monomeric peptide and wherein the SACPs comprise P 11 -30/31 (SEQ ID NOs: 8 and 9).

2 . The SACPs of claim 1 , further comprising a bioactive peptide or a therapeutically active molecule conjugated to the SACPs.

3 . A composition, comprising:
tapes, ribbons, fibrils or fibres, wherein each of the tapes, ribbons, fibrils or fibres has an antiparallel arrangement of SACPs in a β-sheet tape-like substructure, wherein the SACPs comprise P 11 -30/31 (SEQ ID NOs: 8 and 9).

4 . The composition of claim 3 , wherein the composition comprises SACPs which form ribbons and/or fibrils immediately when a first monomeric peptide contacts a second complementary monomeric peptide.

5 . The composition of claim 3 , wherein the SACPs further comprise a bioactive peptide or a therapeutically active molecule conjugated to the SACPs.

6 . The composition of claim 3 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.

7 . The composition of claim 3 , wherein the composition comprises a dental product.

8 . The composition of claim 7 , wherein the dental product is a dental repair, dental treatment, or dental reconstruction product.

9 . The composition of claim 3 , wherein the composition is in the form of a tissue engineering scaffold.

10 . The composition of claim 9 , wherein the scaffold is seeded with cells.

11 . The composition of claim 3 , wherein the composition comprises a tissue repair product.

12 . The composition of claim 3 , wherein the composition comprises a wound healing product.

13 . The composition of claim 3 , wherein the composition comprises a skin treatment.

14 . The composition of claim 3 , wherein the composition comprises a hair care product.

15 . The composition of claim 3 , wherein the composition is part of a bioresponsive and biocompatible surface.

16 . The composition of claim 3 , wherein the composition is a template for nucleation and growth of inorganic materials.

17 . The composition of claim 3 , wherein the composition comprises a continuous filament yarn, staple, floc, cord, or fabric.

18 . The composition of claim 3 , wherein the composition comprises a bone reconstruction product.

Assignees

• Univ Leeds Innovations Ltd

Inventors

• Boden Neville
• Aggeli Amalia
• Ingham Eileen
• Kirkham Jennifer

Classifications

• A61K6/884

  comprising natural or synthetic resins · CPC title

• A61K6/80

  Preparations for artificial teeth, for filling teeth or for capping teeth · CPC title

• A61K6/15

  Compositions characterised by their physical properties · CPC title

• C07K7/06

  having 5 to 11 amino acids · CPC title

• A61K47/42

  Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein (oligopeptides having up to five amino acids {A61K47/183}; polyamino acids A61K47/34) · CPC title