Method for stirring solution

Patent metadata

Field	Value
Publication number	US-9944976-B2
Application number	US-201314381715-A
Country	US
Kind code	B2
Filing date	Feb 27, 2013
Priority date	Feb 29, 2012
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.

First claim

The invention claimed is:

1. A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip comprises a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method comprising:
injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and
rotating said analysis chip to which said test substance-containing solution is injected at a rotation radius of 0.1 mm to 10 mm to apply a centrifugal acceleration of not less than 1×g.

2. The method according to claim 1 , wherein said test substance-containing solution is injected into said recess such that 10% to 70% of said space is left unfilled.

3. The method according to claim 1 , wherein said analysis chip comprises plural recesses to which said test substance-containing solution is injected, said plural recesses being separated by a wall(s) from one another.

4. The method according to claim 1 , wherein said analysis chip is fitted with a cover that covers the entirety of said recess(es); and said test substance-containing solution is sealed in said recess(es).

5. The method according to claim 1 , wherein said analysis chip to which said test substance-containing solution is injected is arranged such that the bottom surface(s) of said recess(es) is/are horizontal or tilted at 0° to 3° with respect to a horizontal plane; and said analysis chip is rotated in a horizontal or tilted direction.

6. A method of analyzing a test substance comprising:
allowing said test substance to bind to a selective binding substance immobilized on an analysis chip by the method of stirring a solution according to claim 1 ; and
detecting said test substance bound to said selective binding substance.

7. The method according to claim 2 , wherein said analysis chip comprises plural recesses to which said test substance-containing solution is injected, said plural recesses being separated by a wall(s) from one another.

8. The method according to claim 2 , wherein said analysis chip is fitted with a cover that covers the entirety of said recess(es); and said test substance-containing solution is sealed in said recess(es).

9. The method according to claim 3 , wherein said analysis chip is fitted with a cover that covers the entirety of said recess(es); and said test substance-containing solution is sealed in said recess(es).

10. The method according to claim 2 , wherein said analysis chip to which said test substance-containing solution is injected is arranged such that the bottom surface(s) of said recess(es) is/are horizontal or tilted at 0° to 3° with respect to a horizontal plane; and said analysis chip is rotated in a horizontal or tilted direction.

11. The method according to claim 3 , wherein said analysis chip to which said test substance-containing solution is injected is arranged such that the bottom surface(s) of said recess(es) is/are horizontal or tilted at 0° to 3° with respect to a horizontal plane; and said analysis chip is rotated in a horizontal or tilted direction.

12. The method according to claim 4 , wherein said analysis chip to which said test substance-containing solution is injected is arranged such that the bottom surface(s) of said recess(es) is/are horizontal or tilted at 0° to 3° with respect to a horizontal plane; and said analysis chip is rotated in a horizontal or tilted direction.

Assignees

• Toray Industries

Inventors

• Kuroda Toshihiko
• Nobumasa Hitoshi

Classifications

• G01N33/5306 Primary

  Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title

• G01N2035/00158

  Elements containing microarrays, i.e. "biochip" · CPC title

• G01N2035/00495

  Centrifuges · CPC title

• G01N33/54366

  Apparatus specially adapted for solid-phase testing · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title