Cholera toxin chimera and its use as a staph vaccine

Patent metadata

Field	Value
Publication number	US-9943582-B2
Application number	US-201414456090-A
Country	US
Kind code	B2
Filing date	Aug 11, 2014
Priority date	Dec 16, 2011
Publication date	Apr 17, 2018
Grant date	Apr 17, 2018

Abstract

The present invention relates to chimeric protein vaccines and methods of use thereof in the treatment of Staphylococcus aureus . One embodiment of the present invention provides a method of generating an immune response in a mammal, that includes administering to the mammal, a composition having a chimeric protein having at least one of: a portion of a cholera toxin, a portion of a heat-labile toxin, and a portion of a shiga toxin; and an antigen having at least one of: an antigenic material from S. aureus and an antigenic material from a S. aureus -specific polypeptide.

First claim

The invention claimed is:

1. A chimeric protein comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 2 or an amino acid sequence that is at least 90% identical to SEQ ID NO: 3 and a truncated iron-regulated surface determinant A (IsdA) protein of S. aureus of SEQ ID NO: 4 as present within the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 6.

2. The chimeric protein of claim 1 and an adjuvant protein selected from the group consisting of cholera toxin, heat-labile toxin and shiga toxin.

3. The chimeric protein of claim 2 , wherein said adjuvant protein is selected from the group consisting of cholera toxin subunit A (CTA), cholera toxin subunit B (CTB), heat-labile toxin subunit B and shiga toxin subunit B.

4. The chimeric protein of claim 3 , wherein said CTA adjuvant is CTA 2 or said CTB adjuvant is CTB.

5. The chimeric protein of claim 4 , wherein said CTA 2 has the amino acid sequence of SEQ ID NO: 2.

6. The chimeric protein of claim 4 , wherein said CTB has the amino acid sequence of SEQ ID NO: 3.

7. The chimeric protein of claim 5 , wherein the chimeric protein has the amino acid sequence of SEQ ID NO: 6.

8. The chimeric protein of claim 6 , wherein the chimeric protein has the amino acid sequence of SEQ ID NO: 5.

9. The chimeric protein of claim 2 , wherein the chimeric protein is a fusion protein.

10. An immunogenic composition comprising the chimeric protein of claim 1 .

11. An immunogenic composition comprising the chimeric protein of claim 7 .

12. An immunogenic composition comprising the chimeric protein of claim 8 .

Assignees

• Univ Boise State

Inventors

• Tinker Juliette

Classifications

• A61P37/04

  Immunostimulants · CPC title

• A61P31/04

  Antibacterial agents · CPC title

• Y10S424/823

  Bacterial vaccine for bovine species, e.g. cattle · CPC title

• A61K2039/543

  intranasal · CPC title

• C07K14/25

  Shigella (G) · CPC title