Transgenic plants having increased tolerance to aluminum

Patent metadata

Field	Value
Publication number	US-9938536-B2
Application number	US-201214355530-A
Country	US
Kind code	B2
Filing date	Nov 1, 2012
Priority date	Nov 2, 2011
Publication date	Apr 10, 2018
Grant date	Apr 10, 2018

Abstract

Methods and materials for modulating aluminum tolerance in plants are disclosed. For example, nucleic acids encoding aluminum tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased tolerance to aluminum and methods of increasing plant yield in soil containing elevated levels of aluminum.

First claim

What is claimed is:

1. A method of increasing plant yield in soil containing elevated levels of Al 3+ , said method comprising growing a plant comprising an exogenous nucleic acid on the soil having the elevated level of Al 3+ , said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 95 percent or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein yield of said plant is increased as compared to the corresponding yield of a control plant that does not comprise said nucleic acid, wherein the regulatory region is heterologous with respect to the nucleotide sequence, and wherein the plant belongs to the Poaceae.

2. A method of increasing plant yield in soil containing elevated levels of Al 3+ , said method comprising growing a plant comprising an exogenous nucleic acid on the soil having the elevated level of Al 3+ , said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence having 95 percent or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO: 1, wherein yield of said plant is increased as compared to the corresponding yield of a control plant that does not comprise said nucleic acid, wherein the regulatory region is heterologous with respect to the nucleotide sequence, and wherein the plant belongs to the Poaceae.

3. The method of claim 1 , wherein said nucleotide sequence encodes a polypeptide having 99 percent or greater sequence identity to said amino acid sequence set forth in SEQ ID NO:2.

4. The method of claim 1 , wherein said nucleotide sequence encodes a polypeptide having 98 percent or greater sequence identity to said amino acid sequence set forth in SEQ ID NO:2.

5. The method of claim 2 , wherein said nucleotide sequence has 99 percent or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:1.

6. The method of claim 2 , wherein said nucleotide sequence has 98 percent or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:1.

7. The method of claim 1 , wherein said nucleotide sequence encodes the polypeptide set forth in SEQ ID NO:2.

8. The method of claim 1 , said method further comprising harvesting biomass from said plant.

9. The method of claim 1 , wherein said regulatory region is a promoter.

10. The method of claim 9 , wherein said promoter is selected from the group consisting of YP0092, PT0676, PT0708, PT0613, PT0672, PT0678, PT0688, PT0837, the napin promoter, the Arcelin-5 promoter, the phaseolin gene promoter, the soybean trypsin inhibitor promoter, the ACP promoter, the stearoyl-ACP desaturase gene promoter, the soybean a′ subunit of /3-conglycinin promoter, the oleosin promoter, the 15 kD zein promoter, the 16 kD zein promoter, the 19 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the Osgt-1 promoter, the beta-amylase gene promoter, the barley hordein gene promoter, p326, YP0144, YP190, p13879, YP0050, p32449, 21876, YP0158, YP0214, YP0380, PT0848, PT0633, the cauliflower mosaic virus (CaMV) 35S promoter, the mannopine synthase (MAS) promoter, the 1′ or 2′ promoters derived from T-DNA of Agrobacterium tumefaciens , the figwort mosaic virus 34S promoter, rice actin promoter, maize ubiquitin-1 promoter, ribulose-1, 5-bisphosphate carboxylase (RbcS) promoter, the pine cab6 promoter, the Cab-1 gene promoter from wheat, the CAB-1 promoter from spinach, the cab1R promoter from rice, the pyruvate orthophosphate dikinase (PPDK) promoter from corn, the tobacco Lhcb1*2 promoter, the Arabidopsis thaliana SUC2 sucrose-H symporter promoter, and a thylakoid membrane protein promoter from spinach, and PT0585.

11. The method of claim 1 , wherein said plant comprising said exogenous nucleic acid has an improved growth rate relative to a corresponding plant that does not comprise said nucleic acid.

12. The method of claim 1 , wherein said plant comprising said exogenous nucleic acid has improved vegetative growth relative to a corresponding plant that does not comprise said nucleic acid.

13. A method of increasing tolerance of a plant to elevated levels of aluminum, said method comprising
a) introducing into a plurality of plant cells an exogenous nucleic acid comprising a regulatory region operably linked to a heterologous nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:2;
b) producing a plant from said plant cell; and
c) growing said plant on soil having an elevated level of Al 3+ , wherein said plant has increased yield as compared to that of a control plan that does not comprise said nucleic acid, and wherein the plant belongs to the Poaceae.

14. A method of increasing tolerance of a plant to elevated levels of aluminum, said method comprising
a) introducing into a plurality of plant cells an exogenous nucleic acid comprising a regulatory region operably linked to a heterologous nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:2; and
b) selecting a plant produced from said plurality of plant cells that has an increased tolerance to elevated A 1 3+ as compared to the tolerance in a corresponding control plant that does not comprise said exogenous nucleic acid, wherein the plant belongs to the Poaceae.

15. The method of claim 14 , wherein regulatory region is a promoter.

16. The method of claim 1 , wherein the plant is a rice plant.

17. The method of claim 1 , wherein the plant is a wheat plant.

18. The method of claim 1 , wherein the plant is a corn plant.

19. The method of claim 1 , wherein the plant is a sorghum plant.

20. The method of claim 1 , wherein the plant is a switchgrass plant.

Assignees

• Ceres Inc

Inventors

• Sosa Julissa
• Wang Wuyi

Classifications

• A23K10/00

  Animal feeding-stuffs · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title

• A23L5/00

  Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor · CPC title

• A23L7/10

  Cereal-derived products · CPC title

• C07K14/415

  from plants · CPC title