Method for extracting lipid from wet biomass

Patent metadata

Field	Value
Publication number	US-9932539-B2
Application number	US-201615364181-A
Country	US
Kind code	B2
Filing date	Nov 29, 2016
Priority date	Sep 7, 2016
Publication date	Apr 3, 2018
Grant date	Apr 3, 2018

Abstract

A method for extracting lipid from wet biomass includes a step in which a wet biomass and a first solvent are mixed to prepare a mixture. The method continues with a step in which said mixture is separated to obtain a solution containing said first solvent and a concentrated biomass. The method continues with a step in which a liquefied gas is added into said concentrated biomass subjected to mechanical dispersion means to obtain lipid.

First claim

What is claimed is:

1. A method for extracting lipid from wet biomass, comprising:
(a) mixing a wet biomass and a first solvent to prepare a mixture;
(b) separating said mixture to obtain a solution containing said first solvent and a concentrated biomass; and
(c) adding a liquefied gas into said concentrated biomass subjected to mechanical dispersion means to obtain lipid.

2. The method of claim 1 , wherein said wet biomass of the step (a) is algae, fungi, animal slurry, or plant slurry.

3. The method of claim 1 , wherein the moisture of said wet biomass of the step (a) is 30 to 99.5 wt % inclusive.

4. The method of claim 1 , wherein said first solvent is methanol, ethanol, isopropanol, propylene glycol, acetone, or a mixture thereof.

5. The method of claim 1 , wherein a mixing volume ratio of said wet biomass to said first solvent is between 1:0.25 and 1:3 inclusive.

6. The method of claim 1 , wherein the moisture of said concentrated biomass of the step (b) is 15 to 95% of the moisture of said wet biomass of the step (a).

7. The method of claim 1 , wherein said liquefied gas of the step (c) is propane, butane, iso-butane, fluorcycloalkane, HFCs refrigerant, HFOs refrigerant, or a mixture thereof.

8. The method of claim 7 , wherein said HFCs refrigerant comprises 1,1,1,2-tetrafluoroethane.

9. The method of claim 7 , wherein said HFOs refrigerant comprises 2,3,3,3-tetrafluoropropene.

10. The method of claim 1 , wherein said mechanical dispersion means of the step (c) comprises using an agitator, a thin-film extractor, or a static mixer.

11. The method of claim 1 , wherein the working pressure of said liquefied gas is between 5 and 100 bar inclusive, and the working temperature thereof is between 15 to 80° C. inclusive.

12. The method of claim 1 , wherein the step (c) further comprises adding a second solvent to extract lipid having a carbon number more than 20, wherein the polarity of said second solvent is greater than the polarity of said liquefied gas, and said second solvent is ethanol, isopropanol, ethyl acetate, acetone, or a mixture thereof.

13. The method of claim 12 , wherein the volume ratio of said second solvent to said liquefied gas is between 1:10 and 1:100 inclusive.

14. A method for extracting lipid from wet biomass, comprising:
(a) mixing a wet cell-disrupted biomass and a first solvent to prepare a mixture;
(b) separating said mixture to obtain a solution containing said first solvent and a concentrated biomass; and
(c) adding a liquefied gas into said concentrated biomass subjected to mechanical dispersion means to obtain lipid.

15. The method of claim 14 , wherein said first solvent is methanol, ethanol, isopropanol, propylene glycol, acetone, or a mixture thereof.

16. The method of claim 14 , wherein said liquefied gas is propane, butane, iso-butane, fluorcycloalkane, HFCs refrigerant, HFOs refrigerant, or a mixture thereof.

17. The method of claim 16 , wherein said HFCs refrigerant comprises 1,1,1,2-tetrafluoroethane.

18. The method of claim 16 , wherein said HFOs refrigerant comprises 2,3,3,3-tetrafluoropropene.

19. The method of claim 14 , wherein said mechanical dispersion means of the step (c) comprises using an agitator, a thin-film extractor, or a static mixer.

Assignees

• Metal Ind Res & Dev Ct

Inventors

• Chen Chi-Hui
• Hung Chun-Hung
• Kuo Tzu-Chen

Classifications

• C11B1/104 Primary

  using super critical gases or vapours · CPC title

• C11B3/006

  by extraction · CPC title

• C11B1/10

  by extracting · CPC title