Method for performing a biochemical analysis, especially in outer space

Patent metadata

Field	Value
Publication number	US-9921214-B2
Application number	US-201313969044-A
Country	US
Kind code	B2
Filing date	Aug 16, 2013
Priority date	Aug 21, 2012
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

The invention is based on a method for performing a biochemical analysis, especially in outer space, wherein at least one analyte in a sample is determined qualitatively and/or quantitatively by means of selective binding of an analyte-specific pair composed of a binding substance and a detection substance to the analyte and by labeling by a labeling substance, and wherein the sample, the binding substance, the detection substance and the labeling substance are mixed in a reaction vessel in one method step. It is proposed that the mixing be brought about by means of mixing bodies.

First claim

The invention claimed is:

1. A method for determining at least one analyte in a sample in outer space under conditions of reduced gravity, comprising:
a) providing, in outer space, a device that comprises a reaction vessel and a magnet unit,
wherein the reaction vessel comprises a detection region and an undetachably fixed capture antibody that binds to the at least one analyte, the undetachably fixed capture antibody located at a spatially predefined position of the reaction vessel within the detection region;
b) mixing together the sample, a detection antibody that binds the at least one analyte, and magnetic inert bodies in the reaction vessel to form a complex composed of analyte, fixed capture antibody, and detection antibody;
wherein the detection antibody carries bound to itself an enzyme capable of catalyzing a reaction that generates a detectable signal,
wherein the magnetic inert bodies consist of magnetic or magnetizable particles that do not substantially bind to the at least one analyte in the sample, to the fixed capture antibody, or to the detection antibody,
wherein the mixing is performed by motion of the magnetic inert bodies caused by application of an alternating magnetic field by the magnet unit,
c) using the magnet unit to position the magnetic inert bodies outside the detection region; and
d) determining the presence of the complex in the reaction vessel by performing optical techniques to measure the detectable signal to determine the at least one analyte in the sample.

2. The method of claim 1 , wherein in step c), the magnetic inert bodies are positioned in a corner of the reaction vessel outside of the detection region.

Assignees

• Astrium Gmbh
• Airbus Ds Gmbh

Inventors

• Kern Peter
• Backes Herbert
• Kubler Ulrich

Classifications

• G01N2035/00336

  Analysers adapted for operation in microgravity, i.e. spaceflight · CPC title

• G01N33/537 Primary

  with separation of immune complex from unbound antigen or antibody · CPC title

• G01N33/54326

  Magnetic particles · CPC title

• G01N33/5306 Primary

  Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding · CPC title