Motor protein device

Patent metadata

Field	Value
Publication number	US-9632082-B2
Application number	US-201214359727-A
Country	US
Kind code	B2
Filing date	Nov 29, 2012
Priority date	Nov 29, 2011
Publication date	Apr 25, 2017
Grant date	Apr 25, 2017

Abstract

[Problem] To provide a motor protein device capable of efficiently transporting and detecting a target antibody.
[Solution] A motor protein device of the present invention includes a collection region where a carrier molecule collects a target molecule using antigen-antibody reaction, and an unloading region where the target molecule is unloaded from the carrier molecule using chemical equilibrium. Further, the motor protein device includes a transport path provided between the collection region and the unloading region through which the carrier molecule can transport the target molecule, and an analysis portion which detects a change in concentration of the target molecule in the unloading region. The unloading region includes certain antibody having capture force higher than predetermined antibody being modified to the carrier molecule and the target molecule is concentrated in the unloading region. The carrier molecule includes actin obtained by modifying the predetermined antibody, and the transport path includes immobilized myosin.

First claim

The invention claimed is:

1. A motor protein device comprising:
a collection region in which a carrier molecule comprising an actin collects a target molecule using an antigen-antibody reaction;
an unloading region in which the target molecule is unloaded from the carrier molecule by using a chemical equilibrium; and
a transport path provided between the collection region and the unloading region, through which the carrier molecule is capable of transporting the target molecule, and in which a myosin as a motor protein is immobilized on the transport path so as to interact with the carrier molecule to move the carrier molecule through the transport path, wherein
the carrier molecule further comprises, as a partial structure, a predetermined antibody capable of binding with a predetermined antigen.

2. A motor protein device comprising:
a collection region in which a carrier molecule comprising an actin collects a target molecule by using an antigen-antibody reaction; and
an unloading region in which a complex comprising the target molecule is unloaded from the carrier molecule by using a chemical equilibrium; and
a transport path provided between the collection region and the unloading region, through which the carrier molecule is capable of transporting the target molecule, and in which a myosin as a motor protein is immobilized on the transport path so as to interact with the carrier molecule to move the carrier molecule through the transport path, wherein
the carrier molecule further comprises, as a partial structure, a predetermined antibody capable of binding with a predetermined antigen.

3. The motor protein device according to claim 1 , wherein the motor protein device is connected to an analysis portion for detecting a change in concentration of the target molecule in the unloading region.

4. The motor protein device according to claim 1 , wherein the unloading region comprises a substance having a target molecule-binding capability higher than that of the predetermined antibody contained in the carrier molecule.

5. The motor protein device according to claim 1 , wherein the carrier molecule comprises a molecule of a modified predetermined antibody, and the transport path comprises the immobilized motor protein throughout a surface thereof.

6. The motor protein device according to claim 1 , wherein the predetermined antibody has binding capability with at least one target molecule.

7. The motor protein device according to claim 3 , wherein the analysis portion comprises a means for electrically detecting the target molecule in the unloading region.

8. The motor protein device according to claim 3 , wherein the analysis portion comprises a means for optically detecting the target molecule in the unloading region.

9. The motor protein device according to claim 1 , wherein the target molecule is a tumor marker.

10. A cancer sensor array comprising the motor protein device according to claim 9 .

11. A motor protein device comprising:
a collection region in which a carrier molecule collects a target molecule using an antigen-antibody reaction;
an unloading region in which the target molecule is unloaded from the carrier molecule by using a chemical equilibrium; and
a transport path provided between the collection region and the unloading region, through which the carrier molecule is capable of transporting the target molecule, and in which a motor protein is immobilized on the transport path so as to interact with the carrier molecule to move the carrier molecule through the transport path,
wherein the transport path is configured to return the carrier molecule to the collection region after unloading the target molecule in the unloading region,
the carrier molecule returning to the collection region after unloading the target molecule in the unloading region is used again for collecting another target molecule in the collection region, and
the carrier molecule comprises, as a partial structure, a predetermined antibody capable of binding with a predetermined antigen.

12. The motor protein device according to claim 11 , wherein the transport path is annularly shaped.

Assignees

• Taiyo Yuden Kk
• Univ Nagaoka Technology

Inventors

• Ishiguro Takashi
• Honda Hajime

Classifications

• G01N33/57565

  involving carcinoembryonic antigen [CEA] · CPC title

• G01N33/558 Primary

  using diffusion or migration of antigen or antibody {(immunochromatographic test strips G01N33/54387)} · CPC title

• G01N33/537 Primary

  with separation of immune complex from unbound antigen or antibody · CPC title

• G01N33/57473

  Physics · mapped topic