Aerobic method of producing alcohols

Patent metadata

Field	Value
Publication number	US-9920334-B2
Application number	US-201615009453-A
Country	US
Kind code	B2
Filing date	Jan 28, 2016
Priority date	Jan 28, 2015
Publication date	Mar 20, 2018
Grant date	Mar 20, 2018

Abstract

A reaction mixture for producing ethanol and/or acetate from a carbon source in aerobic conditions, wherein the mixture comprises
a first acetogenic microorganism in an exponential growth phase; free oxygen; and a second acetogenic microorganism in a stationary phase wherein the first and second acetogenic microorganism is capable of converting the carbon source to the acetate and/or ethanol.

First claim

The invention claimed is:

1. A reaction mixture for producing ethanol and/or acetate from a carbon source in an aerobic condition, the mixture comprising:
a first acetogenic microorganism in an exponential growth phase;
free oxygen; and
a second acetogenic microorganism in a stationary phase,
wherein the first acetogenic microorganism and the second acetogenic microorganism are capable of converting the carbon source to the acetate and/or ethanol.

2. The reaction mixture according to claim 1 , wherein the first and second acetogenic microorganisms are each independently selected from the group consisting of Acetoanaerobium notera (ATCC 35199), Acetonema longum (DSM 6540), Acetobacterium carbinolicum (DSM 2925), Acetobacterium malicum (DSM 4132), Acetobacterium species no. 446, Acetobacterium wieringae (DSM 1911), Acetobacterium woodii (DSM 1030), Alkalibaculum bacchi (DSM 22112), Archaeoglobus fulgidus (DSM 4304), Blautia producta (DSM 2950), Butyribacterium methylotrophicum (DSM 3468), Clostridium aceticum (DSM 1496), Clostridium autoethanogenum (DSM 10061, DSM 19630 and DSM 23693), Clostridium carboxidivorans (DSM 15243), Clostridium coskatii (ATCC no. PTA-10522), Clostridium drakei (ATCC BA-623), Clostridium formicoaceticum (DSM 92), Clostridium glycolicum (DSM 1288), Clostridium ljungdahlii (DSM 13528), Clostridium ljungdahlii C-01 (ATCC 55988), Clostridium ljungdahlii ERI-2 (ATCC 55380), Clostridium ljungdahlii O-52 (ATCC 55989), Clostridium mayombei (DSM 6539), Clostridium methoxybenzovorans (DSM 12182), Clostridium neopropionicum sp, Clostridium ragsdalei (DSM 15248), Clostridium scatologenes (DSM 757), Clostridium species ATCC 29797 , Desulfotomaculum kuznetsovii (DSM 6115), Desulfotomaculum thermobezoicum subsp. thermosyntrophicum (DSM 14055), Eubacterium limosum (DSM 20543), Methanosarcina acetivorans C2A (DSM 2834), Moorella sp. HUC22-1 , Moorella thermoacetica (DSM 521), Moorella thermoautotrophica (DSM 1974), Oxobacter pfennigii (DSM 322), Sporomusa aerivorans (DSM 13326), Sporomusa ovata (DSM 2662), Sporomusa silvacetica (DSM 10669), Sporomusa sphaeroides (DSM 2875), Sporomusa termitida (DSM 4440), and Thermoanaerobacter kivui (DSM 2030).

3. The reaction mixture according to claim 1 , wherein the first acetogenic microorganism has a growth rate of 0.01 to 2 h −1 .

4. The reaction mixture according to claim 1 , wherein the first acetogenic microorganism has an OD 600 of 0.01 to 2.

5. The reaction mixture according to claim 1 , wherein the reaction mixture is made in the aerobic condition by supplying continuous gas flow comprising from 0.000005% to 1% by volume of oxygen to the reaction mixture.

6. The reaction mixture according to claim 1 , wherein the carbon source comprises CO.

7. The reaction mixture according to claim 2 , wherein the first acetogenic microorganism has a growth rate of 0.01 to 2 h −1 .

8. The reaction mixture according to claim 2 , wherein the first acetogenic microorganism has an OD 600 of 0.01 to 2.

9. The reaction mixture according to claim 3 , wherein the first acetogenic microorganism has an OD 600 of 0.01 to 2.

10. The reaction mixture according to claim 2 , wherein the reaction mixture is made in the aerobic condition by supplying continuous gas flow comprising from 0.000005% to 1% by volume of oxygen to the reaction mixture.

11. The reaction mixture according to claim 3 , wherein the reaction mixture is made in the aerobic condition by supplying continuous gas flow comprising from 0.000005% to 1% by volume of oxygen to the reaction mixture.

12. The reaction mixture according to claim 2 , wherein the carbon source comprises CO.

13. The reaction mixture according to claim 1 , wherein the reaction mixture is made in the aerobic condition by supplying continuous gas flow comprising about 0.015% by volume of oxygen to the reaction mixture.

Assignees

• Evonik Degussa Gmbh

Inventors

• Haas Thomas
• Buelter Thomas
• Demler Martin
• Beck Simon

Classifications

• C12P7/02

  containing a hydroxy group · CPC title

• C12R1/145

  Chemistry & Metallurgy · mapped topic

• C12P7/04 Primary

  acyclic · CPC title

• C12P7/065 Primary

  with microorganisms other than yeasts · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title