Automated smear making apparatus

Patent metadata

Field	Value
Publication number	US-9903794-B2
Application number	US-201615348813-A
Country	US
Kind code	B2
Filing date	Nov 10, 2016
Priority date	Dec 28, 2011
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

Abstract

An automated smear making apparatus used to prepare and smear samples on glass slides. In one embodiment, there is provided a smearing subsystem that generally includes a smear cartridge having: an input reel; at least one roll bar; a take-up reel; and a smearing tape. The smearing tape is initially wound within the input reel and coupled to the take-up reel such that the smearing tape can be drawn from the input reel and into the take-up reel. The smearing tape may include a plurality of perforations formed therein. The smearing tape may then be wrapped around the roll bar such that each of the plurality of perforations forms a blade that extends from the smearing tape to expose a smear surface as the smearing tape is drawn into the take-up reel. Alternatively, the smearing tape may be bent such that an edge of the smearing tape forms a smear surface between two roll bars. A slide transport surface is also provided to move a slide across the smear surface.

First claim

What is claimed is:

1. A smearing subsystem for use in an automated slide preparation apparatus, the smearing subsystem comprising:
a smear cartridge comprising:
an input reel;
a take-up reel;
a smearing tape comprising a smear surface included on a blade of a cut-out of the smearing tape, wherein the smearing tape is initially wound within the input reel and coupled to the take-up reel; and
a deflection component configured to deflect the smearing tape cut-out to create the blade that extends from the smearing tape to expose the smear surface as the smearing tape is drawn from the input reel and into the take-up reel; and
a slide transport surface configured to move a slide across the exposed smear surface.

2. The smearing subsystem of claim 1 , wherein the exposed smear surface of the blade forms an acute angle with the slide when the slide transport surface brings the slide in contact with the exposed smear surface such that a sample droplet disposed on the slide contacts the exposed smear surface.

3. The smearing subsystem of claim 2 , further comprising an angle-control mechanism to adjust the acute angle formed between the exposed smear surface of the blade and the slide.

4. The smearing subsystem of claim 3 , wherein the angle-control mechanism is configured to adjust the position of the deflection component with respect to the input reel.

5. The smearing subsystem of claim 3 , wherein the angle-control mechanism is configured to adjust the position of the deflection component with respect to the take-up reel.

6. The smearing subsystem of claim 3 , wherein the angle-control mechanism includes a step motor configured to adjust the deflection component.

7. The smearing subsystem of claim 3 , wherein the angle-control mechanism is configured to adjust the position of the input reel with respect to the deflection component.

8. The smearing subsystem of claim 3 , wherein the angle-control mechanism is configured to adjust the position of the take-up reel with respect to the deflection component.

9. The smearing subsystem of claim 2 , wherein the acute angle is less than about 60 degrees.

10. The smearing subsystem of claim 2 , wherein the acute angle is less than about 45 degrees.

11. The smearing subsystem of claim 2 , wherein the acute angle is less than about 30 degrees.

12. The smearing subsystem of claim 2 , wherein the acute angle is less than about 15 degrees.

13. The smearing subsystem of claim 1 , wherein the deflection component comprises a roll bar.

14. The smearing subsystem of claim 1 , wherein a sample is deposited onto the slide at an aspiration subsystem of the automated slide preparation apparatus, and prior to depositing the sample onto the slide, the slide is transported from a slide holder to the aspiration subsystem.

15. The smearing subsystem of claim 14 , wherein a label to the slide at a printer or a barcode subsystem of the automated slide preparation apparatus, prior to depositing the sample onto the slide.

16. A method for preparing a slide comprising a sample smear using an automated slide preparation apparatus, the method comprising:
depositing a sample droplet onto a slide;
moving the slide towards a smear surface of a smearing tape of a smear cartridge, wherein the smear surface is included on a blade of a cut-out of the smearing tape, and wherein the smearing tape is initially wound within an input reel of the cartridge and coupled to a take-up reel of the smear cartridge;
moving the slide across the smear surface to produce a sample smear, wherein the smear surface is exposed when the position of the blade of the cut-out is extended by a deflection component of the smear cartridge as the smearing tape is drawn from the input reel and into the take-up reel and wherein the smear surface of the blade forms an acute angle with the slide surface on which the sample droplet is disposed; and
drying the sample smear.

17. The method of claim 16 , wherein the sample is deposited onto the slide at an aspiration subsystem of the automated slide preparation apparatus, and prior to depositing the sample onto the slide, the slide is transported from a slide holder to the aspiration subsystem.

18. The method of claim 16 , comprising applying a label to the slide prior to depositing the sample onto the slide.

19. The method of claim 18 , wherein applying a label to the slide comprises printing a label on the slide.

20. The method of claim 19 , wherein the label comprises a barcode.

Assignees

• Abbott Lab

Inventors

• Shoffner John Scott
• Chacko Koshy T
• Solomon Roei

Classifications

• B05C13/02

  for particular articles · CPC title

• G01N2035/00039

  Transport arrangements specific to flat sample substrates, e.g. pusher blade · CPC title

• Y10T436/2575

  Volumetric liquid transfer · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• G01N2035/00138

  Slides · CPC title