Systems and methods for making and processing emulsions

Patent metadata

Field	Value
Publication number	US-9901887-B2
Application number	US-201615282283-A
Country	US
Kind code	B2
Filing date	Sep 30, 2016
Priority date	Apr 7, 2011
Publication date	Feb 27, 2018
Grant date	Feb 27, 2018

Abstract

An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.

First claim

What is claimed:

1. A centrifuge device comprising:
a rotor to receive a centrifuge tube and including a slinger to dispense a fluid to the centrifuge tube;
a lid disposed over the rotor and including an opening;
an adapter secured to the lid in the opening, the adapter including a casing having a cavity and defining a port through the lid, the adapter including a carriage disposed in the cavity of the casing and axially moveable within the casing, the adaptor including a motivator to apply force to the carriage in an axial direction away from the lid, the carriage defining a bore to receive a needle or cannula to extend through the carriage, port, and opening.

2. The centrifuge device of claim 1 , further comprising a packing disposed in the bore of the carriage to secure the needle or cannula.

3. The centrifuge device of claim 1 , wherein the motivator is disposed in the cavity of the casing.

4. The centrifuge device of claim 3 , wherein the carriage defines a recess to receive the motivator.

5. The centrifuge device of claim 1 , wherein the motivator is a spring.

6. The centrifuge device of claim 1 , further comprising a retaining ring to retain the carriage within the cavity of the casing.

7. The centrifuge device of claim 6 , wherein the carriage defines a recess to receive the retaining ring.

8. The centrifuge device of claim 1 , wherein the casing further comprising a second port in fluid communication with the port and the cavity.

9. The centrifuge device of claim 8 , wherein the second port is in fluid communication with the port between the lid and the cavity.

10. The centrifuge device of claim 1 , wherein the adapter is positioned within the lid to dispose an end of the needle or cannula over the slinger, the slinger to receive the fluid from the needle or cannula and distribute the fluid to the centrifuge tube.

11. An integrated apparatus comprising:
an emulsion generator including a carrier fluid port to receive a carrier fluid, a sample vial to receive a sample fluid immiscible with the carrier fluid, a displacement fluid port, and an emulsion outlet port, the emulsion generator including a membrane to generate an emulsion including the carrier fluid and the sample fluid, the emulsion exiting the emulsion generator via the emulsion outlet port, a tube extending from the displacement fluid port into the sample vial;
an amplification device including an inlet port in fluid communication with the emulsion outlet port and to receive the emulsion, the amplification device including a heater to subject the emulsion to amplification conditions and including an effluent port, the amplified emulsion exiting the amplification device via the effluent port; and
a centrifuge device including:
a rotor to receive a centrifuge tube and including a slinger to dispense the amplified emulsion to the centrifuge tube;
a lid disposed over the rotor and including an opening;
an adapter secured to the lid in the opening, the adapter including a casing having a cavity and defining a port through the lid, the adapter including a carriage disposed in the cavity of the casing and axially moveable within the casing, the adaptor including a motivator to apply force to the carriage in an axial direction away from the lid, the carriage defining a bore to receive a needle or cannula to extend through the carriage, port, and opening, the needle or cannula coupled to a tube in fluid communication with the effluent port of the amplification device.

12. The integrated apparatus of claim 11 , wherein the adapter further comprises a packing disposed in the bore of the carriage to secure the needle or cannula.

13. The integrated apparatus of claim 11 , wherein the motivator is disposed in the cavity of the casing.

14. The integrated apparatus of claim 13 , wherein the carriage defines a recess to receive the motivator.

15. The integrated apparatus of claim 11 , wherein the motivator is a spring.

16. The integrated apparatus of claim 11 , wherein the adapter further comprises a retaining ring to retain the carriage within the cavity of the casing.

17. The integrated apparatus of claim 16 , wherein the carriage defines a recess to receive the retaining ring.

18. The integrated apparatus of claim 11 , wherein the casing further comprising a second port in fluid communication with the port and the cavity.

19. The integrated apparatus of claim 18 , wherein the second port is in fluid communication with the port between the lid and the cavity.

20. The integrated apparatus of claim 11 , wherein the adapter is positioned within the lid to dispose an end of the needle or cannula over the slinger, the slinger to receive the amplification from the needle or cannula and distribute the fluid to the centrifuge tube.

Assignees

• Life Technologies Corp

Inventors

• Schultz Jonathan
• Nobile John
• Reed Brian
• Thwar Prasanna
• Roswech Todd
• Sheridan John Andrew

Classifications

• B01F15/00

  Operations & Transport · mapped topic

• B01F3/0807 Primary

  Operations & Transport · mapped topic

• B01L2300/1827

  using resistive heater · CPC title

• B04B5/0414

  comprising test tubes · CPC title

• B01L2300/18

  Means for temperature control · CPC title