Pretreatment apparatus and sample analyzer
US-2017315047-A1 · Nov 2, 2017 · US
System and methods for making and processing emulsions
US9017993B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9017993-B2 |
| Application number | US-201213442547-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 9, 2012 |
| Priority date | Apr 7, 2011 |
| Publication date | Apr 28, 2015 |
| Grant date | Apr 28, 2015 |
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Abstract
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An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
First claim
Opening claim text (preview).
What is claimed is: 1. A system for making membrane-based emulsion droplets, comprising: an emulsion-generating device comprising a housing and a top channel gasket, a bottom channel gasket, and an emulsion-generating membrane disposed between top channel gasket and bottom channel gasket disposed within the housing, a first chamber defined between top channel gasket and emulsion-generating membrane, and a second chamber defined between emulsion-generating membrane and bottom channe…
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- B04B5/0414Primary
Operations & Transport · mapped topic
Operations & Transport · mapped topic
Operations & Transport · mapped topic
- B01F3/0807Primary
Operations & Transport · mapped topic
Operations & Transport · mapped topic
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Frequently asked questions
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- What does patent US9017993B2 cover?
- An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to crea…
- Who is the assignee on this patent?
- Schultz Jonathan, Nobile John, Reed Brian, and 3 more
- What technology area does this patent fall under?
- Primary CPC classification B04B5/0414. Mapped technology areas include Operations & Transport.
- When was this patent published?
- Publication date Tue Apr 28 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).