Method for modulating a bacterial invasion switch

Patent metadata

Field	Value
Publication number	US-9889185-B2
Application number	US-201514853283-A
Country	US
Kind code	B2
Filing date	Sep 14, 2015
Priority date	May 25, 2012
Publication date	Feb 13, 2018
Grant date	Feb 13, 2018

Abstract

The subject matter disclosed herein pertains to the modulation of a bacterial invasion switch and the subsequent use of the bacterium to vaccinate an organism. In one embodiment, the bacterial invasion switch is modulated by changing the proteolysis of ExoR protein. In another embodiment, a mutated bacterium produces a mutant ExoR protein that resists proteolysis.

First claim

What is claimed is:

1. A method for modulating a bacterial invasions switch by changing the proteolysis of ExoR protein, the method comprising administering a protease inhibitor to a bacterium disposed in an aqueous medium such that proteolysis of the mature form of ExoR m is inhibited thereby keeping the bacterium in a non-pathogenic state, wherein the protease inhibitor is a substrate that increases aqueous medium pH or the protease inhibitor is an alkali metal or an alkaline earth metal present in a concentration of at least 2 mM.

2. A method for modulating a bacterial invasions switch by changing the proteolysis of ExoR protein, the method comprising administering a protease inhibitor to a bacterium disposed in an aqueous medium such that proteolysis of the mature form of ExoR m is inhibited thereby keeping the bacterium in a non-pathogenic state, wherein the protease inhibitor is calcium present in a concentration of at least 2 mM.

3. A method for modulating a bacterial invasions switch by changing the proteolysis of ExoR protein, the method comprising administering a protease inhibitor to a bacterium disposed in an aqueous medium such that proteolysis of the mature form of ExoR m is inhibited thereby keeping the bacterium in a non-pathogenic state, wherein the protease inhibitor is sodium present in a concentration of at least 150 mM.

4. The method as recited in claim 1 , wherein the aqueous medium pH is between 6 and 7.

5. The method as recited in claim 1 , wherein the bacterium is S. melilot.

6. The method as recited in claim 2 , wherein the bacterium is S. melilot.

7. The method as recited in claim 3 , wherein the bacterium is S. melilot.

8. The method as recited in claim 4 , wherein the bacterium is S. melilot.

Assignees

• Univ City New York Res Found

Inventors

• Cheng Haiping
• Walcott Shari
• Lu Haiyang
• Luo Li
• Yang Menghua
• Salehi Zahra

Classifications

• A01N37/46

  N-acyl derivatives · CPC title

• A01H17/00

  Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza (lichens A01H15/00) · CPC title

• C12N1/36

  Adaptation or attenuation of cells · CPC title

• C12N9/52

  derived from bacteria {or Archaea} · CPC title

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title