Non-caloric sweeteners and methods for synthesizing
US-9643990-B2 · May 9, 2017 · US
Non-caloric sweeteners and methods for synthesizing
US9850270B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9850270-B2 |
| Application number | US-201514873470-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 2, 2015 |
| Priority date | Oct 3, 2014 |
| Publication date | Dec 26, 2017 |
| Grant date | Dec 26, 2017 |
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- Title
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- Abstract
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Abstract
Official abstract text for this publication.
Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
First claim
Opening claim text (preview).
What is claimed is: 1. A method for synthesizing a mixture of stevioside and rebaudioside KA from rubusoside, the method comprising: preparing an in vitro reaction mixture comprising rubusoside, uridine diphosphate (UDP), uridine diphosphate-glucose (UDP-glucose), and EUGT11 having the sequence of SEQ ID NO: 3, and thereafter, incubating the reaction mixture for a sufficient time to produce a mixture of stevioside and rebaudioside KA, wherein a glucose is covalently coupled to C2′-19-O-glucose of rubusoside to produce rebaudioside KA, and a glucose is covalently coupled to C2′-13-O-glucose of rubusoside to produce stevioside, and wherein the rebaudioside KA has the structure: 2. The method of claim 1 further comprising adding a sucrose synthase to the reaction mixture. 3. The method of claim 2 , wherein the sucrose synthase is selected from the group consisting of an Arabidopsis sucrose synthase 1; an Arabidopsis sucrose synthase 3; and a Vigna radiate sucrose synthase. 4. The method of claim 3 , wherein the sucrose synthase is an Arabidopsis thaliana sucrose synthase 1. 5. The method of claim 2 , wherein the reaction mixture further comprises sucrose. 6. The method of claim 5 , wherein the reaction mixture further comprises a UDP-glycosyltransferase fusion enzyme having the sequence of SEQ ID NO: 11. 7. The method of claim 1 , wherein the reaction mixture further comprises sucrose and a UDP-glycosyltransferase fusion enzyme having the sequence of SEQ ID NO: 11.
Assignees
Inventors
Classifications
Sucrose synthase (2.4.1.13) · CPC title
Polyterpene radicals · CPC title
Glucuronosyltransferase (2.4.1.17) · CPC title
Processes for the preparation of sugar derivatives · CPC title
- C07H15/24Primary
Condensed ring systems having three or more rings · CPC title
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Frequently asked questions
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- What does patent US9850270B2 cover?
- Disclosed are steviol glycosides referred to as rebaudioside V and rebaudioside W. Also disclosed are methods for producing rebaudioside M (Reb M), rebausoside G (Reb G), rebaudioside KA (Reb KA), rebaudioside V (Reb V) and rebaudioside (Reb W).
- Who is the assignee on this patent?
- Conagen Inc
- What technology area does this patent fall under?
- Primary CPC classification C07H15/24. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 26 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).