Devices, apparatus and method for providing photostimulation and imaging of structures

Patent metadata

Field	Value
Publication number	US-9846313-B2
Application number	US-200913121084-A
Country	US
Kind code	B2
Filing date	Sep 25, 2009
Priority date	Sep 25, 2008
Publication date	Dec 19, 2017
Grant date	Dec 19, 2017

Abstract

According to exemplary embodiments of the present disclosure, it is possible to provide method, system, arrangement, computer-accessible medium and device to stimulate individual neurons in brain slices in any arbitrary spatio-temporal pattern, using two-photon uncaging of photo-sensitive compounds such as MNI-glutamate and/or RuBi-Glutamate with beam multiplexing. Such exemplary method and device can have single-cell and three-dimensional precision. For example, by sequentially stimulating up to a thousand potential presynaptic neurons, it is possible to generate detailed functional maps of inputs to a cell. In addition, it is possible to combine this exemplary approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity. Further exemplary embodiments of the present disclosure can include a light-weight, compact portable device providing for uses in a wide variety of applications.

First claim

What is claimed is:

1. A device for affecting at least one radiation, comprising:
a diffractive optical element (DOE); and
at least one spatial light modulator (SLM) arrangement which is structured or configured to affect a user-specified three-dimensional spatial profile of the at least one radiation to trigger at least one of a photo activation, a photo-inactivation or a photo-chemical effect of at least one portion of at least one sample by exciting the at least one portion in a non-linear manner using the at least one radiation.

2. The device of claim 1 , wherein the at least one radiation includes at least one beam, and wherein the at least one SLM arrangement is further structured or configured to split the at least one beam into multiple beamlets so that at least some of which impact the at least one sample.

3. The device according to claim 1 , wherein the at least one sample is at least one of a biological sample, a chemical composition, a semiconductor arrangement or a drug-delivery arrangement.

4. The device according to claim 1 , wherein the at least one SLM arrangement is included in at least one diffractive optical arrangement.

5. The device according to claim 1 , wherein the at least one SLM arrangement affects the at least one radiation using a phase only modulation.

6. The device according to claim 1 , wherein a signal-to-noise ratio of the at least one radiation is greater than about 1.94 fold over a signal from a single radiation system.

7. The device according to claim 1 , wherein the at least one SLM arrangement is configured or structured to at least one of:
provide the at least one effected radiation to a biological sample to provide a photodynamic effect thereto, wherein the at least one effected radiation has an average power that is higher than 100 milliwatts net on the at least one sample,
illuminate microscopic structures within the at least one sample using the at least one radiation, or adjust a phase of the at least one radiation to impact the at least one sample and obtain at least one depth information therefor.

8. The device according to claim 1 , wherein at least a portion of the at least one SLM arrangement is provided in an endoscopic arrangement.

9. The device according to claim 1 , wherein the SLM arrangement is configured or structured to at least one of
(i) illuminate microscopic structures within the at least one sample using the at least one radiation,
(ii) adjust a phase of the at least one radiation to impact the at least one sample and obtain at least one depth information therefor,
(iii) control a delivery of the at least one radiation in a targeted manner by controlling an intensity of the at least one radiation to a particular location on or in the at least one sample, or
(iv) provide the user-specified spatial profile of the at least one radiation on an image plane of the at least one sample.

10. The device according to claim 1 , wherein the at least one radiation includes at least one light radiation, wherein the at least one SLM arrangement is configured or structured to provide the at least one light radiation to the at least one portion of the at least one sample at a depth that is greater than about 1 mm at a particular effective intensity, and wherein the at least one light radiation is provided at the depth within the at least one portion that is based on at least one wavelength of the at least one light radiation.

11. The device according to claim 10 , wherein the at least one light radiation is provided to a specific target at the depth within the at least one portion with an intensity that is greater than that described by I=I 0 exp(σ wl z), wherein I 0 is an original intensity at a surface of the at least one sample, z a depth of a penetration of the at least one radiation, and σ wl is an effective attenuation constant which is a sum of a wavelength dependent average absorption and a wavelength scattering coefficient of a bulk material.

12. The device according to claim 10 , wherein the at least one light radiation is provided to a specific target at the depth within the at least one portion with a modulated intensity which is different from an expected result under predetermined illumination conditions.

13. The device according to claim 1 , wherein the at least one SLM arrangement is structured or configured to effect the at least one radiation to effectuate at least one of (i) a two-photon absorption within the at least one sample, (ii) a three-photon absorption within the at least one sample, or (iii) a Second Harmonics Generation (SHG) associated with the at least one radiation.

14. The device according to claim 1 , wherein the at least one SLM arrangement is a phase-only SLM arrangement which prevents a substantial reduction of intensity of the at least one radiation.

15. The device according to claim 1 , wherein the at least one SLM arrangement includes a single optical component which is solely configured or structured to (i) transmit or reflect and further modify the at least one radiation, (ii) reduce an intensity of the at least one radiation, or (iii) at least partially block the at least one radiation.

16. The device according to claim 1 , wherein the at least one SLM arrangement is included in at least one scanless spatial light modulation (SLM)-based microscope arrangement which affects a coherent light of the at least one radiation.

17. The device according to claim 1 , wherein the at least one SLM arrangement is configured or structured to trigger the photo activation of the at least portion concurrently at multiple specified locations.

18. The device according to claim 1 , wherein the photo-chemical effect comprises at least one of a photo-induced chemical reaction, a photo-isomerisation or a photorelease.

19. The device according to claim 1 , wherein the at least one SLM arrangement is further configured to dynamically control the user-specified spatial profile over time.

20. The device according to claim 1 , wherein the at least one SLM arrangement is further configured to shape the user-specified spatial profile to affect multiple portions of the at least one sample.

21. The device of claim 1 , wherein the user-specified spatial profile depends, at least in part, on a spatial resolution of the device.

22. The device according to claim 1 , wherein the at least one effected radiation has an average power that is higher than an average power of a single radiation system.

23. A device for affecting at least one radiation, comprising:
at least one spatial light modulator (SLM) arrangement which is structured or configured to affect a user-specified three-dimensional spatial profile of the at least one radiation to trigger at least one of a photo activation, a photo-inactivation or a photo-chemical effect of at least one portion of at least one sample by exciting the at least one portion in a non-linear manner using the at least one radiation; and
at least one computer hardware arrangement which is configured to receive data associated with the at least one effected radiation and generate at least one image of the at least one portion of the at least one sample as a function of the data, wherein the at least one image is generated at a duration of the imaging cycle that is less than about 100 ms.

24. A device for affecting at least one radiation, comprising:
at least one spatial light modulator (SLM) arrangement which is structured or configured to affect a user-

Assignees

• Nikolenko Volodymyr
• Yuste Rafael
• Watson Brendon O
• Peterka Darcy
• Univ Columbia

Inventors

• Nikolenko Volodymyr
• Yuste Rafael
• Watson Brendon O
• Peterka Darcy

Classifications

• G02B27/46 Primary

  Systems using spatial filters · CPC title

• G02B21/0004

  specially adapted for specific applications · CPC title

• A61N5/0622

  Optical stimulation for exciting neural tissue · CPC title

• G01N2021/653

  Coherent methods [CARS] · CPC title

• G01N2201/0221

  Portable; cableless; compact; hand-held · CPC title