Methods, systems, and devices for imaging microscopic tumors

Patent metadata

Field	Value
Publication number	US-9820653-B2
Application number	US-201615074614-A
Country	US
Kind code	B2
Filing date	Mar 18, 2016
Priority date	Sep 20, 2013
Publication date	Nov 21, 2017
Grant date	Nov 21, 2017

Abstract

An imager for in vivo viewing of diseased tissue by way of fluorescently conjugated molecules. A generally planar imaging surface with a microlens array. The imager may be modular, such that a plurality of generally planar imaging surfaces can be used to image various aspects of disease tissue simultaneously. Certain implementations include an angle-selective imager, wherein light from substantially perpendicular to the plane of the imager is received, while incident light is selectively eliminated.

First claim

What is claimed is:

1. A system for imaging biological material in a patient, comprising:
a. a fluorescently conjugated molecule capable of binding to the biological material;
b. a light source;
c. a fiber optic light guide;
d. an elongate probe sized for placement inside the cavity of the patient, further comprising at least one substantially planar detection surface, the detection surface further comprising:
i. an imager;
ii. a waveguide in luminary communication with the light source; and
iii. an optical filter; and

e. a visualization system in electrical communication with the probe;

wherein the waveguide is capable of emitting light toward the biological material, such that the biological material's emitted fluorescence is received by the imager for display by way of the visualization system.

2. The system of claim 1 , further comprising a microlens array.

3. The system of claim 1 , wherein the imager further comprises at least one photodiode.

4. The system of claim 3 , further comprising a complementary metal oxide semiconductor process.

5. The system of claim 1 , further comprising a charge-coupled device process.

6. The system of claim 2 , further comprising a waveguide stencil configured to eliminate oblique light from reaching the microlens array.

7. The system of claim 1 , wherein the fluorescently conjugated molecule binds biological material selected from the group consisting of: breast cancer cells, prostate cancer cells, cancer cells inside a tumor bed, cancer cells surrounding a tumor bed, disease cells in the microenvironment surrounding a tumor bed.

8. The system of claim 1 , wherein optical filter is directly patterned on the imager surface.

9. The system of claim 1 , wherein the waveguide further comprises a plurality of optical gratings.

10. The system of claim 1 , further comprising a surgical tool, wherein the elongate probe is operationally coupled with the surgical tool.

11. An angle selective imager, comprising:
a) a light source further comprising a light guide;
b) an elongate probe sized to be positioned within a body cavity of a patient undergoing surgery, wherein the device is operationally coupled with the light source by way of the light guide, said device further comprising at least one substantially planar detection surface, the detection surface further comprising:
i. an imager further comprising a plurality of pixels; and
ii. a microlens array; and

c) a visualization system in electrical communication with the probe capable of computing and displaying fluorescence

wherein the detection surface is adapted to allow light from substantially perpendicular angles from surface to pass through the microlens to the imager, and exclude light incident form other directions.

12. The imager of claim 11 , further comprising a micro-grating.

13. The imager of claim 11 , further comprising a nano-grating.

14. The imager of claim 13 , further comprising at least one cylinder disposed adjacent to the microlens array adjacent to an imager pixel.

15. The imager of claim 14 , further comprising a waveguide, wherein the waveguide is in luminary communication with the light guide.

16. The imager of claim 15 , further comprising an optical filter, wherein the waveguide is capable of emitting light toward fluorescently-tagged cells such that emitted fluorescence is passed through the microlens array and nano-grating and received by the imager.

17. A modular system for imaging fluorescently tagged disease cells, comprising:
a. at least one imager further comprising a plurality of pixels;
b. at least one waveguide further comprising at least one remote light source, wherein the at least one remote light source is capable of emitting light through the at least one waveguide to fluoresce the tagged cells;
c. at least one microlens array configured to direct light to the imager pixels; wherein

the at least one microlens array, imager and waveguide are disposed in a substantially parallel and planar fashion facing the tagged cells so as to transmit light substantially perpendicularly from the tagged cells to the imager by way of the microlens array.

18. The modular system of claim 17 , comprising an optical filter.

19. The modular system of claim 18 , further comprising an additional modular system disposed to face an alternative plane of tagged disease cells.

20. The modular system of claim 18 , wherein the modular system further comprising an angle sensitivity grating.

Assignees

• Univ California

Inventors

• Anwar Moshiur M
• Park Catherine
• Boser Bernard

Classifications

• A61K49/0067

  quantum dots, fluorescent nanocrystals · CPC title

• A61K49/0032

  Methine dyes, e.g. cyanine dyes · CPC title

• A61K49/0058

  Antibodies · CPC title

• A61B5/0084

  for introduction into the body, e.g. by catheters (A61B5/1459 takes precedence) · CPC title

• A61B5/0071 Primary

  by measuring fluorescence emission · CPC title