Compact lens system and array

Patent metadata

Field	Value
Publication number	US-9304280-B2
Application number	US-201414207852-A
Country	US
Kind code	B2
Filing date	Mar 13, 2014
Priority date	Mar 14, 2013
Publication date	Apr 5, 2016
Grant date	Apr 5, 2016

Abstract

A compact lens system for imaging a sample comprising a substrate having a well formed therein. Index matching material is disposed in the well and a lens member is further disposed in the well in optical contact with the index matching material disposed in the well. A spacer member extends from at least one of the substrate and the lower transparent member to define a spacing from a focal point of the lens member, wherein the lens member and index matching material cooperate to image a sample disposed below the lower transparent member.

First claim

What is claimed is:

1. A compact lens system for imaging a sample, said compact lens system comprising:
a substrate having a well defining a first open portion having a first diameter and a second open portion having a second diameter, said second open portion being concentric with said first open portion and in communication therewith, said second diameter being less than said first diameter defining an edge therebetween;
a transparent member adjacent said second open portion of said substrate, said transparent member enclosing said second open portion of said well;
an index matching material being disposed in said well;
a lens member being disposed in at least a portion of said first open portion and said second open portion of said well and in optical contact with said index matching material disposed in said well, a refractive index of said lens member being generally equal to a refractive index of said index matching material; and
a spacer member extending from at least one of said substrate and said transparent member to define a spacing from a focal point of said lens member,
wherein said lens member and said index matching material cooperate to image a sample disposed below said transparent member.

2. The compact lens system according to claim 1 wherein said refractive index of said index matching material is generally equal to a refractive index of said transparent member.

3. The compact lens system according to claim 1 wherein said index matching material is oil and said lens member is in fluid contact with said oil.

4. The compact lens system according to claim 1 wherein said index matching material is a liquid and said lens member is in fluid contact with said liquid.

5. The compact lens system according to claim 1 wherein said index matching material is a monomer that is polymerized within said well to form a solid polymer about at least a portion of said lens member.

6. The compact lens system according to claim 1 wherein said index matching material is a UV curable polymer that is cured within said well to form a solid polymer about at least a portion of said lens member.

7. The compact lens system according to claim 1 wherein said lens member has a refractive index in the range of 1.5 to 1.8 and said index matching material has a refractive index in the range of 1.3 to 1.8.

8. The compact lens system according to claim 1 wherein said lens member is a spherical lens.

9. The compact lens system according to claim 1 wherein a refractive index of said lens member is greater than 1.

10. The compact lens system according to claim 1 , further comprising:
a radiation source output radiation energy for imaging the sample.

11. The compact lens system according to claim 1 , further comprising:
a camera member operably coupled with said lens member for imaging the sample.

12. The compact lens system according to claim 1 wherein said substrate comprises a plurality of wells formed therein, each of said plurality of wells including said index matching material and said lens member.

13. The compact lens system according to claim 12 , further comprising:
a fluidic reservoir for containing at least a portion of said index matching material; and
a fluidic channel fluidly coupling said fluidic reservoir to at least one of said plurality of wells.

14. The compact lens system according to claim 13 wherein said fluidic channel fluidly couples at least a portion of said plurality of wells in series.

15. A compact lens array system for imaging a sample, said compact lens array system comprising:
a substrate having a plurality of microwells disposed in an array of rows and columns, each of the microwells defining a first open portion having a first diameter and a second open portion having a second diameter, said second open portion being concentric with said first open portion and in communication therewith, said second diameter being less than said first diameter defining an edge therebetween;
a transparent member adjacent said second open portion of said substrate, said transparent member enclosing said second open portion of each of said microwells;
a reservoir;
a microfluidic channel fluidly coupling at least one row of said array of microwells to said reservoir;
an index matching material being disposed in said reservoir and in fluid communication with said at least one row of said array of microwells via said microfluidic channel;
a plurality of lens member each being disposed in at least a portion of said first open portion and said second open portion of each of said microwells in optical contact with said index matching material disposed in said microwells, a refractive index of each of said plurality of said lens member being generally equal to a refractive index of said index matching material; and
a spacer member extending from at least one of said substrate and said transparent member to define a spacing from a focal point of said plurality of lens members,
wherein said lens members and said index matching material cooperate to image a sample disposed below said transparent member.

16. The compact lens array according to claim 15 wherein said index matching material is oil and said lens member is in fluid contact with said oil.

17. The compact lens array according to claim 15 wherein said index matching material is a liquid and said lens member is in fluid contact with said liquid.

18. The compact lens array according to claim 15 wherein said lens member has a refractive index in the range of 1.5 to 1.8 and said index matching material has a refractive index in the range of 1.3 to 1.8.

19. The compact lens array according to claim 15 , further comprising:
a fluidic reservoir for containing at least a portion of said index matching material; and
a fluidic channel fluidly coupling said fluidic reservoir to at least one of said wells,
wherein said fluidic channel fluidly couples a plurality of said wells in series.

Assignees

• Univ Michigan

Inventors

• Gulari Mayurachat Ning
• Ghannad-Rezaie Mostafa
• Tripathi Anurag
• Chronis Nikolaos

Classifications

• G02B7/027 Primary

  the lens being in the form of a sphere or ball · CPC title

• G02B3/0056

  arranged along two different directions in a plane, e.g. honeycomb arrangement of lenses (G02B3/0043 takes precedence; miniaturised objectives for electronic devices employing wafer level optics G02B13/0085) · CPC title

• G01N15/1436

  the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell · CPC title

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title