Microparticle measuring apparatus

Patent metadata

Field	Value
Publication number	US-9816913-B2
Application number	US-201615231146-A
Country	US
Kind code	B2
Filing date	Aug 8, 2016
Priority date	Oct 15, 2012
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

A microparticle measuring apparatus for highly accurately detecting the position of a microparticle flowing through a flow channel includes a light irradiation unit for irradiating a microparticle flowing through a flow channel with light, and a scattered light detection unit for detecting scattered light from the microparticle, including an objective lens for collecting light from the microparticle, a light splitting element for dividing the scattered light from the light collected by the objective lens, into first and second scattered light, a first scattered light detector for receiving an S-polarized light component, and an astigmatic element disposed between the light splitting element and the first scattered light detector, and making the first scattered light astigmatic. A relationship between a length L from a rear principal point of the objective lens to a front principal point of the astigmatic element, and a focal length f of the astigmatic element satisfies the following formula I.

1.5 f≦L ≦2.5 f   (I)

First claim

The invention claimed is:

1. An apparatus, comprising:
a light source configured to irradiate a microparticle with first light;
an objective lens configured to collect second light emitted from the microparticle;
a scattered light detector configured to receive scattered third light from the second light collected by the objective lens; and
an astigmatic element between the objective lens and the scattered light detector,
wherein the astigmatic element is configured to make the scattered third light astigmatic,
wherein a relationship between a length L from a rear principal point of the objective lens to a front principal point of the astigmatic element and a focal length f of the astigmatic element satisfies the following formula (I)
1.5 f≦L≦ 2.5 f   (I).

2. The apparatus according to claim 1 , further comprising a light splitting element configured to divide the scattered third light from the second light collected by the objective lens into a fourth scattered light and a fifth scattered light,
wherein the fourth scattered light is received by the scattered light detector.

3. The apparatus according to claim 2 , wherein the light splitting element is a polarization element,
wherein the polarization element is configured to diffract the scattered third light into an S-polarized light component and a P-polarized light component,
wherein the fourth scattered light is the S-polarized light component.

4. The apparatus according to claim 1 , wherein the astigmatic element is a cylindrical lens.

5. The apparatus according to claim 4 , wherein the astigmatic element is positioned on a plane perpendicular to a v-direction based on:
a light input direction to the microparticle defined as a z-direction,
a direction of flow of the microparticle defined as a x-direction, and
a direction perpendicular to the z-direction and the x-direction defined as the y-direction,
wherein the astigmatic element is further configured to have an angle of 0° to 5° between a generatrix of the astigmatic element and a first line that extends in the x-direction or a second line that extends in the z-direction.

6. The apparatus according to claim 1 , wherein the scattered light detector comprises a light receiving surface divided into a plurality of areas.

7. The apparatus according to claim 6 , wherein the scattered light detector is a quadrant photodiode.

8. The apparatus according to claim 7 , wherein the scattered light detector is further configured on a plane perpendicular to a y-direction based on:
a light input direction to the microparticle defined as a z-direction,
a direction of flow of the microparticle defined as a x-direction, and
a direction perpendicular to the z-direction and the x-direction defined as the y-direction,
wherein the scattered light detector is further configured to have an angle of 40° to 50° between a first line that divides the scattered light detector and a second line that extends in the x-direction or a third line that extends in the z-direction.

9. The apparatus according to claim 1 , wherein a first position of flow of the microparticle is detected based on a change in second position of reception of the scattered third light.

10. The apparatus according to claim 9 , wherein
a light receiving surface of the scattered light detector is divided into a lattice shape to have a first area, a second area, a third area, and a fourth area, and
the scattered light detector is further configured to acquire positional information of the microparticle based on a difference (A-C) between a detection value A of the first area and a detection value C of the third area that is separately positioned from the first area.

11. The apparatus according to claim 9 , wherein
a light receiving surface of the scattered light detector is divided into a lattice shape to have a first area, a second area, a third area, and a fourth area, and
the scattered light detector is further configured to acquire positional information of the microparticle based on a difference ((A+C)−(B+D)) between a sum (A+C) of a detection value A of the first area and a detection value C of the third area that is separately positioned from the first area, and a sum (B+D) of a detection value B of the second area adjacent to the first area and a detection value D of the fourth area that is separately positioned from the second area.

12. A flow cytometer, comprising:
a light source configured to irradiate a microparticle with first light, wherein the microparticle flows through a flow channel;
an objective lens configured to collect second light emitted from the microparticle;
a scattered light detector configured to receive third scattered light from the second light collected by the objective lens, wherein the third scattered light is scattered by the microparticle; and
an astigmatic element between the objective lens and the scattered light detector,
wherein the astigmatic element is configured to make the third scattered light astigmatic,
wherein a relationship between a length L from a rear principal point of the objective lens to a front principal point of the astigmatic element and a focal length f of the astigmatic element satisfies the following formula (I)
1.5 f≦L≦ 2.5 f   (I).

13. The flow cytometer according to claim 12 , wherein the light source is further configured to irradiate the microparticle with the first light, wherein the microparticle flows through an analysis chip,
wherein the analysis chip includes the flow channel.

14. The flow cytometer according to claim 12 , further comprising a light splitting element configured to divide the third scattered light from the second light that is collected by the objective lens into a fourth scattered light and a fifth scattered light, wherein the fifth scattered light is received by the scattered light detector.

15. The flow cytometer according to claim 14 , wherein the light splitting element is a polarization element, wherein the polarization element is configured to diffract the third scattered light into an S-polarized light component and a P-polarized light component, wherein the fourth scattered light is the S-polarized light component.

16. The flow cytometer according to claim 12 , wherein the astigmatic element is a cylindrical lens.

17. The flow cytometer according to claim 12 , wherein the scattered light detector comprises a light receiving surface divided into a plurality of areas.

18. The flow cytometer according to claim 17 , wherein the scattered light detector is a quadrant photodiode.

19. The flow cytometer according to claim 12 , wherein a first position of flow of the microparticle is detected based on a change in second position of reception of the third scattered light.

20. The flow cytometer according to claim 19 , wherein
a light receiving surface of the scattered light detector is divided into a lattice shape to have a first area, a second area, a third area, and a fourth area, and
the scattered light detector is further configured to acquire positional information of the microparticle based on a difference (A-C) between a detection value A of the first area and a detection value C of the third area that is separately positioned from the first area.

Assignees

• Sony Corp

Inventors

• Takeuchi Taichi
• Imanishi Shingo

Classifications

• G01N2015/1006

  for cytology · CPC title

• G01N15/1436 Primary

  the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell · CPC title

• G01N2201/0683

  Brewster plate; polarisation controlling elements · CPC title

• G01N21/49

  within a body or fluid · CPC title

• G01N15/1434

  Optical arrangements · CPC title