Disposable direct capture device

Patent metadata

Field	Value
Publication number	US-9816901-B2
Application number	US-201314438961-A
Country	US
Kind code	B2
Filing date	Dec 13, 2013
Priority date	Jan 31, 2013
Publication date	Nov 14, 2017
Grant date	Nov 14, 2017

Abstract

The invention relates to a liquid sample preparation device such as a disposable, collapsible, flexible polymeric bag containing an adsorptive curtain of a functionalized shaped polymeric fiber bed for the direct capture of biomolecules from liquid samples. The functionalized shaped polymeric fiber bed includes fibrillated, ridged or winged-shaped fiber structures that significantly increases the surface area of the fiber resulting in enhanced separation, retention and/or purification of liquid samples containing biomolecules of interest as the liquid samples contact the adsorptive curtain of functionalized shaped fibers. Liquid samples include unclarified liquid feeds or other liquids containing one or more biomolecules of interest, including, but not limited to vaccines, recombinant proteins, cells, stem cells, monoclonal antibodies (mAbs), proteins, antibody, peptides, oligopeptides, nucleic acids, oligonucleotides, RNA, DNA, oligosaccharides and polysaccharides.

First claim

What is claimed is:

1. A disposable flexible liquid sample preparation device 20 comprising:
a collapsible body 30 having an internal compartment 32 defined by sidewalls 34 , an interior surface 36 , an exterior surface 38 , an inlet 52 , an outlet 54 , and an adsorbent polymeric element 40 contained within the internal compartment, the adsorbent polymeric element comprising a continuous, shaped monofilament fiber assembled in loops substantially parallel to each other each of the loops having a first end 42 and a second end 44 wherein the first end and the second end are attached to an adsorbent polymeric bonding element 46 , 48 , wherein the adsorbent polymeric bonding elements are attached to opposing peripheries of the internal compartment.

2. The device of claim 1 , wherein the body is a bag.

3. The device of claim 1 , wherein the adsorbent polymeric element comprises a polymeric shaped fiber bed.

4. The device of claim 3 , wherein the shaped fiber further comprises surface pendant functional groups that provide cation-exchange or anion-exchange functionality.

5. The device of claim 3 , wherein the shaped fiber is meltspun.

6. The device of claim 3 , wherein the shaped fiber has a cross-section with a middle region comprising a longitudinal axis that runs down the center of the fiber and having a plurality of projections that extend radially outwardly from the from the middle region.

7. The device of claim 3 , wherein the shaped fiber comprises polyolefins, thermoplastic polymers, polypropylene, polyester, polyethylene, polyamide, copolyesters, liquid crystalline polymers, thermoplastic elastomers, thermoplastic polyurethane elastomers polyethers, polyether esters, PBAX elastomeric olefins or mixtures thereof.

8. A method of making a disposable flexible liquid sample preparation device ( 20 ) comprising:
a) providing two polymeric sheets, each sheet having an outer periphery;
b) providing an adsorbent polymeric element ( 40 ) comprising a continuous, shaped monofilament fiber, assembled in loops substantially parallel to each other, each of the loops having a first end 42 and a second end 44 wherein the first end and the second end are attached to at least one adsorbent polymeric bonding element ( 46 or 48 ), the bonding element having first and second ends;
c) placing the adsorbent polymeric element and bonding element between the two polymeric sheets such that the outer peripheries of the two sheets overlap each other and the first and second ends of the bonding element are sandwiched between opposing overlapping outer peripheries;
d) bonding together the two polymeric sheets at the overlapping outer peripheries and the first and second ends of the bonding element forming a device having an internal compartment for housing the adsorbent polymeric element fixedly attached to opposing outer peripheries of the two polymeric sheets.

9. The method of claim 8 , wherein an outlet and an inlet are fixedly attached to the device.

10. A method of making a disposable flexible liquid sample preparation device comprising:
a) providing a single polymeric sheet having an outer periphery;
b) providing an adsorbent polymeric element comprising a continuous, shaped monofilament fiber, assembled in loops substantially parallel to each other, each of the loops having a first end and a second end, wherein the first end and the second end are attached to at least one adsorbent polymeric bonding element, the bonding element having first and second ends;
c) placing the adsorbent polymeric element and bonding element on the polymeric sheet;
d) folding the polymeric sheet such the outer periphery of the sheet overlap and the first and second ends of the bonding element are sandwiched between opposing overlapping outer peripheries;
e) bonding the polymeric sheet at the overlapping outer periphery and the first and second ends of the bonding element;
f) forming a device having an internal compartment for housing the adsorbent polymeric element fixedly attached to opposing outer peripheries of the polymeric sheet.

11. The method of claim 10 , wherein an outlet and an inlet are fixedly attached to the device.

Assignees

• Emd Millipore Corp

Inventors

• Cacace Benjamin

Classifications

• B01J47/022

  characterised by the construction of the column or container · CPC title

• G01N1/405 Primary

  by adsorption or absorption · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title

• B01L2300/0681

  Filter · CPC title

• B01L3/505

  Flexible containers without fluid transport within · CPC title