Device for guiding cell migration and guiding method implementing such a device

Patent metadata

Field	Value
Publication number	US-9803173-B2
Application number	US-201214345517-A
Country	US
Kind code	B2
Filing date	Sep 17, 2012
Priority date	Sep 19, 2011
Publication date	Oct 31, 2017
Grant date	Oct 31, 2017

Abstract

The subject matter of the present invention is a device for guiding cell migration comprising a substrate having a textured surface intended to be brought into contact with cells, said textured surface having an anisotropic three-dimensional structure consisting of a network of projections inclined relative to the normal to the plane formed by said textured structure, in the direction imparted by said anisotropic structure.
The invention also concerns, according to another aspect, a method for guiding cell migration including the bringing into contact of cells with a substrate having a textured surface and an anisotropic three-dimensional structure, said structure consisting of projections inclined as previously described.
The device or method according to the invention can in particular be applied in the fields of dermatology, implantology and tissue engineering.

First claim

The invention claimed is:

1. A method for guiding cell migration, comprising bringing the cells into contact with a substrate having a textured surface which has an anisotropic three-dimensional structure, said structure consisting of a network of projections inclined relative to the normal to the plane formed by said textured surface, in the direction imparted by said anisotropic structure, said projections having a regular spacing which is smaller than the diameter of the cells, wherein the cells are conveyed on a support surface, and wherein said support surface and/or said textured surface comprises one or more additional protuberances which make it possible to control the distance between the two said surfaces.

2. The guiding method as claimed in claim 1 , wherein the support surface is an artificial surface or a surface of the natural environment of said cells.

3. The guiding method as claimed in claim 1 , wherein the cells are confined between said support surface and said textured surface.

4. The method as claimed in claim 1 , wherein the distance between said support surface and said textured surface is between 0 and 10 μm.

5. The guiding method as claimed in claim 1 , wherein the additional protuberances are in the form of pillars with a diameter of between 100 and 500 μm, and a height of less than 10 μm.

6. The guiding method as claimed in claim 2 , wherein the artificial surface is selected from the group consisting of a gel cell culture surface, a glass coverslip, and the inside of a microfluidic channel.

7. The guiding method as claimed in claim 2 , wherein the surface of the natural environment of said cells is selected from the group consisting of the surface of a living tissue and the surface of a wound.

8. The method as claimed in claim 1 , wherein the distance between said support surface and said textured surface is between 3 and 6 μm.

9. The method as claimed in claim 5 , wherein the pillars have a height of between 3 and 6 μm.

10. The method as claimed in claim 1 , wherein said network of projections has a spacing which is less than 20 μm.

11. The method as claimed in claim 1 , wherein said network of projections has a spacing between 0.1 and 15 μm.

12. The method as claimed in claim 1 , wherein said network of projections has a spacing between 5 and 10 μm.

13. The method as claimed in claim 1 , wherein said network of projections has a spacing of about 5 μm.

14. The method as claimed in claim 1 , wherein the inclined projections have an angle of inclination of less than or equal to 45° relative to the normal to the plane formed by said textured surface.

15. The method as claimed in claim 1 , wherein the inclined projections have an angle of inclination of between 10° and 45° relative to the normal to the plane formed by said textured surface.

16. The method as claimed in claim 1 , wherein the inclined projections have an aspect ratio, corresponding to the ratio of the height to the diameter of the projections, of between 0.5 and 20.

17. The method as claimed in claim 1 , wherein the inclined projections have an aspect ratio, corresponding to the ratio of the height to the diameter of the projections of between 2 and 10.

18. The method as claimed in claim 1 , wherein the inclined projections are in the shape selected from the group consisting of cylinders having a spherical or ovoid cross section, cones, pyramids, lamellae having a cross section shape selected from a rectangle and a parallelogram, and flakes having a substantially triangular, semielliptical or semicircular shape.

19. The method as claimed in claim 1 , wherein the inclined projections are in the shape of cylinders with a diameter of between 10 nm and 10 μm.

20. The method as claimed in claim 19 wherein the cylinders have a diameter of between 0.5 and 3 μm.

21. The method as claimed in claim 1 , wherein the substrate having a textured surface is adhesive for cells.

22. The method as claimed in claim 1 , wherein the substrate having a textured surface is non-adhesive for cells.

23. The method as claimed in claim 22 , wherein the non-adhesive substrate consists of a non-adhesive material selected from the group consisting of a fluoropolymer and a material made non-adhesive by chemical treatment through the grafting of molecules of polyethylene glycol (PEG).

24. The method as claimed in claim 1 , wherein the substrate comprises a dressing, an implant, a prosthesis, an artificial-tissue support, a microfluidic channel, or a Lab-on-a-chip device integrating channels.

25. The method as claimed in claim 24 wherein said substrate comprises a dressing.

Assignees

• Inst Curie
• Centre Nat De La Rech Scientifique—Cnrs
• Soc De Dev Et De Rech Ind

Inventors

• Le Berre Mael
• Piel Matthieu
• Chen Yong
• Liu Yanjun

Classifications

• C12N2533/30

  Synthetic polymers · CPC title

• C12N2533/32

  Polylysine, polyornithine · CPC title

• C12N5/0068 Primary

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• B29C39/42

  Casting under special conditions, e.g. vacuum · CPC title

• C12N5/0656

  Adult fibroblasts · CPC title