Reactive oxidative species generating materials and methods of use

Patent metadata

Field	Value
Publication number	US-9789186-B2
Application number	US-201615377104-A
Country	US
Kind code	B2
Filing date	Dec 13, 2016
Priority date	Aug 31, 2012
Publication date	Oct 17, 2017
Grant date	Oct 17, 2017

Abstract

Materials capable of delivering stabilized free radicals to targeted treatment sites. The materials comprise semi-crystalline, hydrolytically degradable polymers that are subjected to ionizing radiation to create stabilized free radicals therein. Upon exposure to oxygen containing aqueous media, the materials generate reactive oxidative species which are useful in biological processes.

First claim

What is claimed is:

1. A method of stimulating blood vessel formation comprising:
applying at least one semi-crystalline, hydrolytically degradable polymeric material comprising stabilized free radicals to a treatment site, wherein the polymeric material has been subjected to ionizing radiation at a dose that exceeds that required for sterilization but is less than that required to substantially degrade the polymeric material,
wherein the at least one semi-crystalline, hydrolytically degradable polymeric material comprises a first polymeric material and a second polymeric material, and
wherein the first polymeric material and the second polymeric material have been subjected to different doses of ionizing radiation.

2. The method of claim 1 wherein the dose of ionizing radiation is from about 30 kGy to about 50 kGy.

3. A method of stimulating blood vessel formation comprising:
applying at least one semi-crystalline, hydrolytically degradable polymeric material comprising stabilized free radicals to a treatment site, wherein the polymeric material has been subjected to ionizing radiation at a dose of less than 50 kGy and is sterilized by non-ionizing radiation methods,
wherein the at least one semi-crystalline, hydrolytically degradable polymeric material comprises a first polymeric material and a second polymeric material, and
wherein the first polymeric material and the second polymeric material have been subjected to different doses of ionizing radiation.

4. The method of claim 1 , wherein the polymeric material, upon contact with an aqueous media, enables multi-phasic generation of reactive oxidative species.

5. The method of claim 1 , wherein an initial burst of reactive oxidative species production and a subsequent sustained period of reactive oxidative species production occurs upon contacting the polymeric material with an aqueous media.

6. The method of claim 1 , wherein the first polymeric material has a different hydrolytic degradation rate than the second polymeric material.

7. The method of claim 1 , wherein the first polymeric material has a different degree of crystallinity than the second polymeric material.

8. The method of claim 1 , wherein the first and second polymeric materials each comprise stabilized free radicals.

9. The method of claim 1 , wherein the first polymeric material comprises stabilized free radicals and the second polymeric material does not contain stabilized free radicals.

10. The method of claim 1 , wherein the first polymeric material comprises a different amount of stabilized free radicals than the second polymeric material.

11. The method of claim 1 , wherein the first and second polymeric materials have different reactive oxidative species generation profiles.

12. The method of claim 1 , wherein at least one of the first and second polymeric materials is bioabsorbable.

13. The method of claim 12 , wherein the bioabsorbable polymer is selected from poly(dioxanone), poly(glycolide), poly(lactide) poly(ε-caprolactone), poly(anhydrides) such as poly(sebacic acid), poly(hydroxyalkanoates) such as poly(3-hydroxybutyrate), copolymers of any of these and combinations thereof.

14. The method of claim 1 , wherein the dose of ionizing radiation is less than about 50 kGy.

15. The method of claim 1 , wherein the biocompatible material further comprises at least one therapeutically active agent.

16. The method of claim 1 , wherein the polymeric material has been sterilized by a non-ionizing radiation method.

17. The method of claim 3 , wherein the polymeric material, upon contact with an aqueous media, enables multi-phasic generation of reactive oxidative species.

18. The method of claim 3 , wherein an initial burst of reactive oxidative species production and a subsequent sustained period of reactive oxidative species production occurs upon contacting the polymeric material with an aqueous media.

19. The method of claim 3 , wherein the first polymeric material has a different hydrolytic degradation rate than the second polymeric material.

20. The method of claim 3 , wherein the first polymeric material has a different degree of crystallinity than the second polymeric material.

21. The method of claim 3 , wherein the first and second polymeric materials each comprise stabilized free radicals.

22. The method of claim 3 , wherein the first polymeric material comprises stabilized free radicals and the second polymeric material does not contain stabilized free radicals.

23. The method of claim 3 , wherein the first polymeric material comprises a different amount of stabilized free radicals than the second polymeric material.

24. The method of claim 3 , wherein the first and second polymeric materials have different reactive oxidative species generation profiles.

25. The method of claim 3 , wherein at least one of the first and second polymeric materials is bioabsorbable.

26. The method of claim 25 , wherein the bioabsorbable polymer is selected from poly(dioxanone), poly(glycolide), poly(lactide) poly(ε-caprolactone), poly(anhydrides) such as poly(sebacic acid), poly(hydroxyalkanoates) such as poly(3-hydroxybutyrate), copolymers of any of these and combinations thereof.

27. The method of claim 3 , wherein the dose of ionizing radiation is from about 30 kGY to about 50 kGy.

28. The method of claim 3 , wherein the biocompatible material further comprises at least one therapeutically active agent.

Assignees

• Gore & Ass

Inventors

• Brown Tiffany J
• Lafleur Adam S
• Mazich Kenneth
• Towler Jeffrey C
• Zhang Ji

Classifications

• A61L31/06

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds {(A61L31/041 takes precedence)} · CPC title

• A61L31/148 Primary

  Materials at least partially resorbable by the body · CPC title

• A61L27/34 Primary

  Macromolecular materials · CPC title

• A61L27/58

  Materials at least partially resorbable by the body · CPC title

• A61L31/10

  Macromolecular materials · CPC title