Unified detection system for fluorometry, luminometry and spectrometry

Patent metadata

Field	Value
Publication number	US-9784670-B1
Application number	US-201514602189-A
Country	US
Kind code	B1
Filing date	Jan 21, 2015
Priority date	Jan 22, 2014
Publication date	Oct 10, 2017
Grant date	Oct 10, 2017

Abstract

The devices and systems disclosed herein provide multiple optical capabilities in a single device or system. Methods for using these devices and systems are provided. These devices and systems are configurable for operation in each of a spectroscopy mode, a fluorescence mode, and a luminescence mode, and are capable of performing spectroscopic, fluorescence, and luminescence observations, measurements, and analyses when operated in the corresponding spectroscopy mode, fluorescence mode, or luminescence mode. These devices and systems include mirror dispersion elements having multiple faces including an optical dispersion element on one face (e.g., a diffraction grating or a prism) and a reflective element on another face (e.g., a mirror). These multiple capabilities eliminate the need to move or load a sample in multiple devices when subjecting a sample to multiple analyses, and thus provide greater accuracy, precision, and speed while reducing complexity and cost of sample analysis.

First claim

The invention claimed is:

1. A unified detection device for fluorometry, luminometry, and spectrometry, comprising:
a first optical element comprising a diffractive surface movably mounted on a first movable mount;
a first mirror configured for reflecting light after contact of said light with said diffractive surface;
a first lens configured for focusing or for collimating light reflected from said first mirror;
a second mirror movably mounted on a second movable mount;
a second optical element movably mounted on a third movable mount, said second optical element having a first face and a second face, said first face having a reflective surface configurable to reflect light from said second mirror, and said second face having a diffractive surface configurable to diffract light from said second mirror;
a second lens configured for directing light to or onto said second optical element, wherein the second lens is positioned to collect light off-axis relative to a direct light path from the first lens to the second mirror; and
a photodetector receiving light from the second optical element;
wherein the second mirror has a first configuration to reflect light from the first lens to the second optical element and a second configuration to reflect light from the first lens in a direction away from the second optical element.

2. The unified detection device of claim 1 , further comprising a sample holder configured to hold a sample for optical observation, measurement, or analysis.

3. The unified detection device of claim 1 , further comprising a sample handling device configured to transport a sample or a sample holder to a location suitable for optical observation, measurement, or analysis.

4. The unified detection device of claim 3 , wherein i) said sample handling device comprises a fluid handling device or system configured to transport a sample or a sample holder, or ii) said sample handling device is configured to operate with a fluid handling device or system configured to transport a sample or a sample holder.

5. The unified detection device of claim 1 , wherein said diffractive surface comprises a grating or a prism.

6. A system for fluorometry, luminometry, and spectrometry comprising a unified detection device of claim 1 , a communication device, and a communication channel operably connecting said device with said communication device.

7. The system for fluorometry, luminometry, and spectrometry of claim 6 , further comprising a sample handling device configured to transport a sample or a sample holder to a location suitable for optical observation, measurement, or analysis.

8. The system for fluorometry, luminometry, and spectrometry of claim 7 , wherein said sample handling device is configured to operate with a fluid handling device or system configured to transport a sample.

Assignees

• Theranos Inc

Inventors

• Mohan Karan
• Anekal Samartha

Classifications

• G01N21/76

  Chemiluminescence; Bioluminescence · CPC title

• G02B27/42

  Diffraction optics {, i.e. systems including a diffractive element being designed for providing a diffractive effect}(G02B27/60 takes precedence) · CPC title

• G02B5/18

  Diffraction gratings {(holographic optical elements G02B5/32, G03H; integrally combined with optical fibres G02B6/02057; for coupling light guides G02B6/34; integrally combined with optical integrated light guides G02B6/12; grating systems G02B27/44)} · CPC title

• G02B5/04

  Prisms · CPC title

• G01N21/6486

  Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence) · CPC title