Promoter, promoter control elements, and combinations, and uses thereof

Patent metadata

Field	Value
Publication number	US-9777285-B2
Application number	US-201414476566-A
Country	US
Kind code	B2
Filing date	Sep 3, 2014
Priority date	Feb 1, 2008
Publication date	Oct 3, 2017
Grant date	Oct 3, 2017

Abstract

The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels in plants, and plants containing such promoters or promoter control elements.

First claim

What is claimed is:

1. A vector construct comprising:
a) a first nucleic acid molecule comprising a promoter consisting of the nucleotide sequence of SEQ ID NO: 9 or a nucleotide sequence with at least 95 percent sequence identity to SEQ ID NO:9, wherein said promoter has the promoter activity of SEQ ID NO:9; and
b) a second nucleic acid molecule to be expressed, wherein said first nucleic acid molecule and said second nucleic acid molecule are heterologous to each other and are operably linked.

2. The vector construct according to claim 1 , wherein said first nucleic acid molecule comprises the nucleic acid sequence of SEQ ID NO: 9.

3. The vector construct according to claim 1 , wherein said second nucleic acid molecule encodes a polypeptide.

4. The vector construct according to claim 3 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in the sense orientation.

5. The vector construct according to claim 4 , wherein said second, nucleic acid molecule is transcribed into an RNA molecule that expresses the polypeptide encoded by said second nucleic acid molecule.

6. The vector construct according to claim 1 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in the antisense orientation.

7. The vector construct according to claim 6 , wherein transcription of said second nucleic acid molecule produces an antisense molecule.

8. The vector construct according to claim 1 , wherein transcription of said second nucleic acid molecule produces an RNAi molecule against an endogenous gene.

9. The vector construct according to claim 3 , wherein said second nucleic acid molecule encodes a polypeptide of agronomic interest, wherein the polypeptide of agronomic interest provides a desirable characteristic associated with plant morphology, physiology, growth and development, yield, nutritional enhancement, disease or pest resistance, environmental tolerance, or chemical tolerance.

10. A plant or plant cell comprising the vector construct according to claim 1 .

11. A plant or plant cell stably transformed with the vector construct according to claim 1 .

12. A transgenic seed of the plant according to claim 10 .

13. A method of directing transcription comprising combining, in an environment suitable for transcription:
a) a first nucleic acid molecule comprising a promoter consisting of the nucleotide sequence of SEQ ID NO: 9 or a nucleotide sequence with at least 95 percent sequence identity to SEQ ID NO:9, wherein said promoter has the promoter activity of SEQ ID NO:9; and
b) a second nucleic acid molecule; wherein said first nucleic acid molecule and said second nucleic acid molecule are heterologous to each other and operably linked, and transcribing said second nucleic acid molecule.

14. A method of expressing an exogenous coding region in a plant comprising:
a) transforming at least one plant cell with the vector of claim 1 ;
b) regenerating at least one stably transformed plant from the at least one transformed plant cell of step (a); and
c) selecting at least one plant containing the transformed plant cell, wherein expression of the second nucleic acid molecule results in production of a polypeptide encoded by said second transcribable nucleic acid molecule.

15. A method of altering the expression of a gene in a plant comprising:
a) transforming a plant cell with the vector construct according to claim 1 , and
b) regenerating a stably transformed plant from said transformed plant cell.

16. A plant prepared according to the method of claim 14 .

17. A transgenic seed from the plant according to claim 16 .

18. A method of producing a transgenic plant, said method comprising:
a) transforming a plant cell with the vector according to claim 1 ; and
b) growing a plant from said plant cell.

19. The method of claim 18 , wherein said second nucleic acid molecule comprises a nucleic acid sequence encoding a polypeptide.

20. The method of claim 18 , wherein said second nucleic acid molecule is operably linked to said first nucleic acid molecule in the antisense orientation.

21. The method of claim 18 , wherein transcription of said second nucleic acid molecule produces an RNAi molecule.

22. A plant or plant cell comprising the vector construct according to claim 2 .

23. A plant or plant cell stably transformed with the vector construct according to claim 2 .

24. A transgenic seed of the plant according to claim 22 .

25. A method of producing a transgenic plant, said method comprising:
a) introducing into a plant cell the vector according to claim 2 ; and
b) growing a plant from said plant cell.

Assignees

• Ceres Inc

Inventors

• Schneeberger Richard
• Margolles-Clark Emilio
• Tatarinova Tatiana
• Fang Yiwen
• Alexandrov Nickolai
• Medrano Leonard
• Pennell Roger
• Theiss Noah
• Grizard Danielle
• Davis Shauna J
• Robles Dennis
• Portereiko Michael

Classifications

• C12N15/8222

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8237

  Externally regulated expression systems · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title