Polypeptides having dextranase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9765409-B2
Application number	US-201414782162-A
Country	US
Kind code	B2
Filing date	Apr 4, 2014
Priority date	Apr 5, 2013
Publication date	Sep 19, 2017
Grant date	Sep 19, 2017

Abstract

The present invention relates to isolated polypeptides having dextranaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

First claim

The invention claimed is:

1. A method for reducing viscosity in a sugar solution comprising contacting the sugar solution with an isolated polypeptide having dextranase activity, wherein the amino acid sequence of the polypeptide has at least 92% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

2. The method of claim 1 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

3. The method of claim 1 , wherein the amino acid sequence of the polypeptide has at least 97% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

4. The method of claim 1 , wherein the amino acid sequence of the polypeptide has at least 98% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

5. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of amino acids 17 to 594 of SEQ ID NO: 2.

6. The method of claim 1 , wherein the polypeptide is a fragment of the amino acid sequence of amino acids 17 to 594 of SEQ ID NO: 2, wherein the fragment has dextranase activity.

7. The method of claim 1 , wherein the polypeptide is contacted with the sugar solution before a clarification step, before an evaporator step, in holding juice tanks, and/or in syrup tanks.

8. A recombinant microbial host cell comprising a polynucleotide encoding a polypeptide having dextranase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in the host cell and wherein the amino acid sequence of the polypeptide has at least 92% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

9. The recombinant host cell of claim 8 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

10. The recombinant host cell of claim 8 , wherein the amino acid sequence of the polypeptide has at least 97% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

11. The recombinant host cell of claim 8 , wherein the amino acid sequence of the polypeptide has at least 98% sequence identity to the sequence of amino acids 17 to 594 of SEQ ID NO: 2.

12. The recombinant host cell of claim 8 , wherein the polypeptide comprises the amino acid sequence of amino acids 17 to 594 of SEQ ID NO: 2.

13. The recombinant host cell of claim 8 , wherein the polypeptide is a fragment of the amino acid sequence of amino acids 17 to 594 of SEQ ID NO: 2, wherein the fragment has dextranase activity.

14. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 8 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

15. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 9 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

16. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 10 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

17. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 11 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

18. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 12 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

19. A method of producing a polypeptide having dextranase activity, comprising:
(a) cultivating the host cell of claim 13 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.

20. The method of claim 14 , wherein the host cell is a filamentous fungal host cell.

Assignees

• Novozymes As

Inventors

• Landvik Sara
• Palmen Lorena G
• Basso Thiago O
• Da Silva Eduardo Alberto Borges

Classifications

• C12N9/96

  Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title

• C13B20/002 Primary

  using microorganisms or enzymes · CPC title

• C12N9/2454 Primary

  Dextranase (3.2.1.11) · CPC title

• C12Y302/01011

  Dextranase (3.2.1.11) · CPC title