Non-caloric sweetener
US-2016208225-A1 · Jul 21, 2016 · US
Non-caloric sweetener
US9765104B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9765104-B2 |
| Application number | US-201615016725-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 5, 2016 |
| Priority date | May 5, 2014 |
| Publication date | Sep 19, 2017 |
| Grant date | Sep 19, 2017 |
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- Title
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- Abstract
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- Assignees and inventors
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- First independent claim
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- CPC / IPC classifications
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Abstract
Official abstract text for this publication.
Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for synthesizing the rebaudioside of formula I: the method comprising: (a) preparing a reaction mixture comprising: (i) rebaudioside E, uridine diphosphate-glucose (UDP-glucose), and an EUGT11 UDP glycosyltransferase having the amino acid sequence of SEQ ID NO: 1; or (ii) rebaudioside E, sucrose, an EUGT11 UDP glycosyltransferase having the amino acid sequence of SEQ ID NO: 1, a sucrose synthase, and either or both of uridine diphosphate (UDP) and UDP-glucose; (b) incubating the reaction mixture for a sufficient time to produce the rebaudioside of formula (I), wherein a glucose is covalently coupled to the rebaudioside E to produce the rebaudioside of formula (I); and (c) purifying the rebaudioside of formula (I) produced from the reaction mixture to a purity level of from 50% to 100% by weight. 2. The method of claim 1 , wherein the reaction mixture (i) is used for synthesizing the rebaudioside of formula (I). 3. The method of claim 1 , wherein the reaction mixture (ii) is used for synthesizing the rebaudioside of formula (I). 4. The method of claim 1 , wherein the sucrose synthase is an Arabidopsis sucrose synthase 1 having the amino acid sequence of SEQ ID NO: 3. 5. A method for synthesizing the rebaudioside of formula I: the method comprising: (a) preparing a reaction mixture comprising: (i) rebaudioside E, uridine diphosphate-glucose (UDP-glucose), and a UDP-glycosyltransferase fusion enzyme comprising a UDP-glycosyltransferase domain coupled to a sucrose synthase domain, the UDP glycosyltransferase fusion enzyme comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 5 and having UDP-glycosyltransferase and sucrose synthase activities; or (ii) rebaudioside E, sucrose, a UDP-glycosyltransferase fusion enzyme, and either or both of UDP and UDP-glucose, wherein the UDP-glycosyltransferase fusion enzyme comprises a UDP-glycosyltransferase domain coupled to a sucrose synthase domain, the UDP glycosyltransferase fusion enzyme comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 5 and having UDP-glycosyltransferase and sucrose synthase activities; (b) incubating the reaction mixture for a sufficient time to produce the rebaudioside of formula (I), wherein a glucose is covalently coupled to the rebaudioside E to produce the rebaudioside of formula (I); and (c) purifying the rebaudioside of formula (I) produced from the reaction mixture to a purity level of from 50% to 100% by weight. 6. The method of claim 5 , wherein the reaction mixture (i) is used for synthesizing the rebaudioside of formula (I). 7. The method of claim 5 , wherein the reaction mixture (ii) is used for synthesizing the rebaudioside of formula (I). 8. The method of claim 5 , wherein the UDP glycosyltransferase domain is a UDP-glycosyltransferase domain of an EUGT11 UDP glycosyltransferase having the amino acid sequence of SEQ ID NO: 1. 9. The method of claim 5 , wherein the sucrose synthase domain is a sucrose synthase domain of an Arabidopsis sucrose synthase 1 having the amino acid sequence of SEQ ID NO: 3.
Assignees
Inventors
Classifications
Hexosyltransferases (2.4.1) · CPC title
- C07H15/256Primary
Polyterpene radicals · CPC title
- C07H15/24Primary
Condensed ring systems having three or more rings · CPC title
Glycosyltransferases (2.4) · CPC title
Sucrose synthase (2.4.1.13) · CPC title
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Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9765104B2 cover?
- Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five β-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.
- Who is the assignee on this patent?
- Conagen Inc, Conagen Inc
- What technology area does this patent fall under?
- Primary CPC classification C07H15/256. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 19 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 4 related publications on this page (citations in our corpus or others sharing the same primary CPC).