Cobalamin acquisition protein and use thereof

Patent metadata

Field	Value
Publication number	US-9758550-B2
Application number	US-201514958321-A
Country	US
Kind code	B2
Filing date	Dec 3, 2015
Priority date	Apr 30, 2012
Publication date	Sep 12, 2017
Grant date	Sep 12, 2017

Abstract

The present invention relates to a cobalamin acquisition protein, compositions containing the cobalamin acquisition protein, and the use of such compositions.

First claim

What is claimed is:

1. A pharmaceutical composition comprising a protein that binds Vitamin B 12 , said protein comprising an amino acid sequence having at least 90% identity with SEQ ID NO: 2, and a pharmaceutically acceptable excipient.

2. The composition of claim 1 , wherein the protein binds Vitamin B 12 with an affinity less than 10 −5 M.

3. The composition of claim 1 , wherein the composition is a liquid, solid, cream, or paste.

4. The composition of claim 1 , wherein the protein binds Vitamin B 12 with an affinity from about 10 −5 M to about 10 −12 M.

5. The composition of claim 1 , wherein the composition, when administered to a subject, is capable of reducing the growth and/or proliferation of a microorganism in or on the subject.

6. The composition of claim 1 , wherein the protein comprises from about 0.1% (w/w) to about 90% (w/w) of the pharmaceutical composition.

7. The composition of claim 6 , wherein the protein comprises from about 0.5% (w/w) to about 30% (w/w) of the pharmaceutical composition.

8. A method of treating a bacterial infection in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the composition of claim 1 .

9. The method of claim 8 , wherein the composition is administered topically or orally to the subject.

10. The method of claim 8 , wherein the composition is administered non-parenterally to the subject.

Assignees

• Woods Hole Oceanographic Inst

Inventors

• Saito Makoto
• Bertrand Erin M

Classifications

• G01N33/82 Primary

  involving vitamins {or their receptors} · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title

• C07K14/43504

  from invertebrates · CPC title

• C07K14/00 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title

• C07K14/405

  from algae · CPC title