Cobalamin acquisition protein and use thereof

Patent metadata

Field	Value
Publication number	US-9234012-B2
Application number	US-201313874437-A
Country	US
Kind code	B2
Filing date	Apr 30, 2013
Priority date	Apr 30, 2012
Publication date	Jan 12, 2016
Grant date	Jan 12, 2016

Abstract

The present invention relates to a cobalamin acquisition protein, compositions containing the cobalamin acquisition protein, and the use of such compositions.

First claim

What is claimed is:

1. A recombinant microorganism comprising a heterologous nucleic acid encoding a protein comprising an amino acid sequence having at least 90% identity with SEQ ID NO: 2, wherein the nucleic acid is disposed within a recombinant expression vector construct and can be expressed to produce a protein that binds Vitamin B 12 .

2. The recombinant microorganism of claim 1 , wherein the nucleic acid is operatively associated with an inducible promoter.

3. The recombinant microorganism of claim 1 , wherein the nucleic acid is operatively associated with a constitutive promoter.

4. The recombinant microorganism of claim 1 , wherein the microorganism, under the same environmental conditions, (i) is capable of binding more vitamin B 12 over a preselected period of time than an organism without the nucleic acid, (ii) is capable of taking up more Vitamin B 12 over a preselected period of time than an organism without the nucleic acid, (iii) is capable of growing faster over a preselected period of time than an organism without the nucleic acid, or a combination thereof.

5. The recombinant microorganism of claim 1 , wherein the microorganism is an algae.

6. A viable culture comprising the recombinant microorganism of claim 1 .

7. A method of growing the culture of claim 6 under conditions that permit the growth of the recombinant microorganism.

Assignees

• Woods Hole Oceanographic Inst

Inventors

• Saito Makoto
• Bertrand Erin Marie

Classifications

• G01N33/82 Primary

  involving vitamins {or their receptors} · CPC title

• C07K14/405 Primary

  from algae · CPC title

• C07K14/43504

  from invertebrates · CPC title

• A61K38/16

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof {(enzyme inhibitors A61K38/005)} · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title