Electric-field imager for assays

Patent metadata

Field	Value
Publication number	US-9753028-B2
Application number	US-201615147103-A
Country	US
Kind code	B2
Filing date	May 5, 2016
Priority date	May 5, 2015
Publication date	Sep 5, 2017
Grant date	Sep 5, 2017

Abstract

This disclosure describes an electric-field imaging system and method of use. In accordance with implementations of the electric-field imaging system, a fluid sample can be placed on top of a pixel-based impedance sensor. An image of the target analytes can be created immediately afterwards. From this image, computer imaging algorithms can determine attributes (e.g., size, type, morphology, volume, distribution, number, concentration, or motility, etc.) of the target analytes. The electric-field imaging sensor can be used for a variety of agglutination or agglomeration assays.

First claim

What is claimed is:

1. A system for investigating one or more target analytes, comprising:
a fluid sample for an agglutination or agglomeration assay;
an impedance-based sensor including a plurality of metal panels that define an active sensor area with a sensor pitch that is higher frequency than a Nyquist spatial sampling rate suitable for detecting a smallest member of a group of one or more target analytes in the fluid sample; and
a processor configured to receive electrical signals associated with a change in impedance or charge from the plurality of metal panels when the fluid sample is placed on the active sensor area, the processor further configured to generate at least one image frame based on the electrical signals received from the plurality of metal panels.

2. The system of claim 1 , wherein the agglutination or agglomeration assay comprises an immunoassay to be sensed by the impedance-based sensor.

3. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a kinetic agglutination assay to be sensed by the impedance-based sensor.

4. The system of claim 1 , wherein the agglutination or agglomeration assay comprises an agglomeration of beads assay to be sensed by the impedance-based sensor.

5. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a kinetic agglomeration of beads assay to be sensed by the impedance-based sensor.

6. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a coagulation assay to be sensed by the impedance-based sensor.

7. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a kinetic coagulation assay to be sensed by the impedance-based sensor.

8. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a surface antigen assay to be sensed by the impedance-based sensor.

9. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a receptor assay from biopsy to be sensed by the impedance-based sensor.

10. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a circulating blood cell assay to be sensed by the impedance-based sensor.

11. The system of claim 1 , wherein the agglutination or agglomeration assay comprises a circulating nucleic acid assay to be sensed by the impedance-based sensor.

12. The system of claim 1 ,
wherein the at least one image frame includes multiple image frames collected at different times.

13. The system of claim 12 , wherein processor is further configured to determine one or more attributes of the one or more target analytes in the fluid sample for the agglutination or agglomeration assay based on the multiple image frames collected at different times.

14. The system of claim 13 , wherein the one or more attributes determined by the processor include one or more of: size, type, morphology, volume, distribution, number, concentration, or motility of the one or more target analytes.

15. The system of claim 1 , wherein the one or more target analytes comprise functionalized beads.

16. A method of performing an assay, comprising:
placing a fluid sample on an active sensor area of an impedance-based sensor, wherein the active sensor area is defined by a plurality of metal panels, the fluid sample containing one or more target analytes for an agglutination or agglomeration assay;
detecting changes in impedance or charge based on electrical signals generated by the plurality of metal panels;
generating a plurality of images based upon detected changes in impedance or charge caused by the one or more target analytes in the fluid sample over a period of time; and
determining one or more attributes of the one or more target analytes in the fluid sample for the agglutination or agglomeration assay based on the plurality of images generated over the period of time.

17. The method of claim 16 , wherein the agglutination or agglomeration assay comprises at least one of: an immunoassay to be sensed by the impedance-based sensor; a kinetic agglutination assay to be sensed by the impedance-based sensor; an agglomeration of beads assay to be sensed by the impedance-based sensor; a kinetic agglomeration of beads assay to be sensed by the impedance-based sensor; a coagulation assay to be sensed by the impedance-based sensor; a kinetic coagulation assay to be sensed by the impedance-based sensor; a surface antigen assay to be sensed by the impedance-based sensor; a receptor assay from biopsy to be sensed by the impedance-based sensor; a circulating blood cell assay to be sensed by the impedance-based sensor; or a circulating nucleic acid assay to be sensed by the impedance-based sensor.

18. The method of claim 16 , wherein the one or more attributes include one or more of: size, type, morphology, volume, distribution, number, concentration, or motility of the one or more target analytes.

19. The method of claim 16 , wherein the one or more target analytes comprise functionalized beads.

20. An impedance-based sensor, comprising:
a plurality of metal panels that define an active sensor area configured to receive a fluid sample for an agglutination or agglomeration assay; and
a processor configured to receive electrical signals associated with a change in impedance or charge from the plurality of metal panels when the fluid sample is placed on the active sensor area, the processor further configured to generate at least one image frame based on the electrical signals received from the plurality of metal panels.

Assignees

• Maxim Integrated Products

Inventors

• Koo Ronald B
• Grage Henry

Classifications

• G01N15/1031

  by measuring electrical or magnetic effects · CPC title

• G01N33/5302 Primary

  Apparatus specially adapted for immunological test procedures · CPC title

• G01N33/4836

  using multielectrode arrays · CPC title

• G01N33/487

  of liquid biological material · CPC title