Tethering of confactors on graphene-like materials

Patent metadata

Field	Value
Publication number	US-9742011-B2
Application number	US-201414776777-A
Country	US
Kind code	B2
Filing date	Mar 17, 2014
Priority date	Mar 15, 2013
Publication date	Aug 22, 2017
Grant date	Aug 22, 2017

Abstract

A family of customizable tethering molecules for tethering cofactors such as, but not necessarily limited to, nicotinamine adenine dinucleotide (NAD+/NADH, NAD(P)+/NAD(P)H) to substrates or structures formed from or including graphene-like materials is described. The tethered cofactor can then be used, for example, as biosensors employed for clinical diagnostic, food industry, medical drug development and environmental and military applications, as well as in reagentless biofuel cells for power generation.

First claim

What is claimed is:

1. A complex formed from a NAD/(P)+/NAD(P)H cofactor tethered to a graphene-like material selected from the group consisting of multi-walled carbon nanotubes, single walled carbon nanotubes, graphene, rGO, and other graphene-based or graphene-containing substrates, wherein the cofactor is attached, via a covalent bond, to a pyrene butanoic acid succinimidyl ester tethering molecule which is attached to the graphene-like material via π-π stacking.

2. The complex of claim 1 wherein the graphene-like material is a multi-walled carbon nanotube (MWCNT).

3. The complex of claim 1 further comprising a protein which requires the presence of the cofactor for biological function tethered to the graphene-like material.

4. The complex of claim 3 wherein the protein is an enzyme.

5. The complex of claim 3 wherein the protein is a biocatalyst.

6. A method for forming a biological complex comprising:
covalently attaching a NAD(P)+/NAD(P)H cofactor to a pyrene butanoic acid succinimidyl ester tethering enzyme and;
attaching the tethering enzyme to a graphene-like material selected from the group consisting of multi-walled carbon nanotubes, single walled carbon nanotubes, graphene, rGO, and other graphene-based or graphene-containing substrates via π-π stacking.

7. The method of claim 6 further comprising tethering a protein that interacts with the NAD(P)+/NAD(P)H confactor to the graphene-like material.

8. The method of claim 7 wherein the protein is an enzyme.

9. The method of claim 7 wherein the protein is a biocatalyst.

Assignees

• Atanassov Plamen B
• Narvaez Villarrubia Claudia W
• Garcia Sergio Omar
• Stc Unm

Inventors

• Atanassov Plamen B
• Narvaez Villarrubia Claudia W
• Garcia Sergio Omar

Classifications

• C12P19/36

  Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate · CPC title

• C01B2202/06

  Multi-walled nanotubes · CPC title

• C12N11/02

  Enzymes or microbial cells immobilised on or in an organic carrier · CPC title

• C01B31/0253

  Chemistry & Metallurgy · mapped topic

• H01M4/90

  Selection of catalytic material · CPC title