Membrane transfer method and tool

Patent metadata

Field	Value
Publication number	US-9732371-B2
Application number	US-201314036339-A
Country	US
Kind code	B2
Filing date	Sep 25, 2013
Priority date	Jan 14, 2010
Publication date	Aug 15, 2017
Grant date	Aug 15, 2017

Abstract

A method for transferring a filter membrane ( 130 ) bearing a retentate to a reagent pad ( 310 ), in which the membrane is mounted in a support frame ( 120 ) having a shoulder ( 121 ) and a skirt ( 122 ) projecting from the shoulder, the pad being mounted on a cassette ( 300 ) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

First claim

The invention claimed is:

1. A tool for transferring a filter membrane bearing a retentate to a reagent pad, comprising:
a tool body with a free end having a region adapted to receive a reagent cassette having the reagent pad mounted thereon,
an ejection head mounted to said tool body and movable translationally about said tool body between a first position in which said region is unoccupied by said ejection head and a second position in which said region is occupied by said ejection head thereby serving to eject said cassette from said region when said cassette is received by said tool body,
an actuating lever operatively connected to said ejection head to translate said ejection head from said first position to said second position,
a seal disposed on the tool body so as to sealingly retain the cassette on the tool body when said ejection head is in said first position, and
an opening in said tool body for the application of a pressure difference between the cassette and the body of the tool.

2. A tool according to claim 1 , wherein said opening in said tool body is adapted to be connected to a vacuum pump that is external to the tool, and opens into a fluid-tight space between the cassette and the body of the tool.

3. A tool according to claim 1 , wherein the ejection head is annular.

4. A tool according to claim 1 , further comprising an integrated pressure regulator.

5. A tool according to the claim 4 , wherein the integrated pressure regulator is tared for a pressure between 40 and 70 millibars.

6. A tool according to claim 1 , wherein the seal disposed on the tool body so as to sealingly retain the cassette on the tool body inserted into a groove formed in the body for that purpose.

Assignees

• Emd Millipore Corp

Inventors

• Waiche Gael
• Kukuczka Monika

Classifications

• C12M33/02 Primary

  by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029) · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• C12M33/14

  with filters, sieves or membranes · CPC title