Membrane transfer method and tool

Patent metadata

Field	Value
Publication number	US-9315850-B2
Application number	US-201113521066-A
Country	US
Kind code	B2
Filing date	Jan 12, 2011
Priority date	Jan 14, 2010
Publication date	Apr 19, 2016
Grant date	Apr 19, 2016

Abstract

A method for transferring a filter membrane ( 130 ) bearing a retentate to a reagent pad ( 310 ), in which the membrane is mounted in a support frame ( 120 ) having a shoulder ( 121 ) and a skirt ( 122 ) projecting from the shoulder, the pad being mounted on a cassette ( 300 ) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

First claim

The invention claimed is:

1. A method of transferring a filter membrane bearing a retentate to a reagent pad, wherein:
the membrane is mounted in a support frame having a shoulder and a skirt projecting from the shoulder;
the pad having a membrane contact surface that is substantially flat, the pad being mounted on a cassette of complementary form to said skirt, the pad not being a gel growth medium;
the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder;

the method comprising:
sealingly engaging the skirt of the membrane support frame on the cassette;
establishing a predetermined pressure difference between the cassette and the membrane support frame such that the membrane takes the form of a dome pointing towards the pad, said pressure difference being a pressure reduction applied in a space between the membrane and the cassette;
while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the membrane contact surface of the pad;
maintaining a force on the frame so as to make the frame move downward until it comes into abutment formed by the shoulder while maintaining the pressure difference, thereby uniformly transferring said filter membrane to said membrane contact surface of the pad.

2. A method according to claim 1 , wherein it is preceded by saturating the pad with a reagent.

3. A method according to claim 2 , wherein the reagent is a solution adapted to reveal the microorganisms contained in the retentate of the membrane.

4. A method according to claim 1 , wherein said dome has a region comprising a central contact area, and wherein said maintenance of force on said frame transfers said membrane filter progressively from said central contact area radially outwards.

5. A method according to claim 1 , wherein said predetermined pressure ranges from 40 to 70 millibars.

6. A method according to claim 1 , wherein said pad is impregnated with a liquid.

7. A method according to claim 6 , wherein said liquid is a reagent adapted to reveal the presence of microorganisms in said retentate.

Assignees

• Waiche Gael
• Kukuczka Monika
• Emd Millipore Corp

Inventors

• Waiche Gael
• Kukuczka Monika

Classifications

• C12M33/14

  with filters, sieves or membranes · CPC title

• C12Q1/24 Primary

  Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms · CPC title

• C12M33/02 Primary

  by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029) · CPC title