Light microscope and microscopy method
US-11966036-B2 · Apr 23, 2024 · US
US9727024B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9727024-B2 |
| Application number | US-201314441270-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 7, 2013 |
| Priority date | Nov 12, 2012 |
| Publication date | Aug 8, 2017 |
| Grant date | Aug 8, 2017 |
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A phase distribution is calculated such that modulated light has a predetermined intensity distribution on a target plane and displayed on a phase modulation plane, readout light enters the phase modulation plane so as to generate the modulated light. When calculating the phase distribution, a region on the phase modulation plane is divided into N regions A 1 . . . A N , with sizes set such that integration values of an intensity distribution in the regions are equal to each other. Further, a region on the target plane is divided into N regions B 1 . . . B N , with sizes set such that integration values of an intensity distribution in the regions are equal to each other. The phase distribution is calculated by obtaining an optical path length from the region A n to the region B n , and determining the phase of the region A n based on the optical path length.
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The invention claimed is: 1. A phase modulation method, using a spatial light modulator comprising a phase modulation plane including a plurality of two-dimensionally arrayed modulation regions, for modulating readout light, of which a readout light intensity distribution in a first section including a first optical axis of the readout light is axially symmetric with respect to the first optical axis, in phase in each of the plurality of modulation regions to generate modulated light, comprising: a phase distribution calculation step of calculating a phase distribution to be displayed on the phase modulation plane such that the modulated light has a predetermined intensity distribution on a target plane separated by a predetermined optical distance from the phase modulation plane; and a modulated light generation step of displaying the phase distribution on the phase modulation plane, and causing the readout light to enter the phase modulation plane so as to generate the modulated light, wherein the phase distribution calculation step comprises: a step of dividing a light entering region on the phase modulation plane which the readout light enters into N (N is an integer of 2 or more) concentric first regions A 1 . . . A N centered on the first optical axis of the readout light and setting sizes of the first regions A 1 . . . A N such that first integration values of the readout light intensity distribution in the first section including the first optical axis of the readout light, the first integration values being obtained by integrating the readout light intensity distribution in the respective first regions A 1 . . . A N , are equal to each other, and dividing a target region on the target plane into N concentric second regions B 1 . . . B N centered on a second optical axis of the modulated light and setting sizes of the second regions B 1 . . . B N such that second integration values of a modulated light intensity distribution in a second section including the second optical axis of the modulated light, the second integration values being obtained by integrating the modulated light intensity distribution in the respective second regions B 1 . . . B N , are equal to each other; and a step of calculating the phase distribution by obtaining an optical path length L n from the first region A n to the second region B n (n is each integer from 1 to N), and determining a phase of the first region A n based on the optical path length L n . 2. The phase modulation method according to claim 1 , wherein the phase distribution calculation step further comprises a measurement step of measuring the readout light intensity distribution in the first section including the first optical axis of the readout light which enters the phase modulation plane. 3. The phase modulation method according to claim 1 , wherein optical components are not provided on an optical path of the modulated light from the phase modulation plane to the target plane. 4. A phase modulation apparatus comprising: a light source configured to output readout light of which a readout light intensity distribution in a first section including a first optical axis of the readout light is axially symmetric with respect to the first optical axis; a spatial light modulator comprising a phase modulation plane including a plurality of two-dimensionally arrayed modulation regions, and configured to modulate the readout light in phase in each of the plurality of modulation regions to generate modulated light; and a phase distribution computation unit configured to calculate a phase distribution to be displayed on the phase modulation plane such that the modulated light has a predetermined intensity distribution on a target plane separated by a predetermined optical distance from the phase modulation plane, wherein the phase distribution computation unit calculates the phase distribution to be displayed on the phase modulation plane by dividing a light entering region on the phase modulation plane which the readout light enters into N (N is an integer of 2 or more) concentric first regions A 1 . . . A N centered on the first optical axis of the readout light and setting sizes of the first regions A 1 . . . A N such that first integration values of the readout light intensity distribution in the first section including the first optical axis of the readout light, the first integration values being obtained by integrating the readout light intensity distribution in the respective first regions A 1 . . . A N , are equal to each other, dividing a target region on the target plane into N concentric second regions B 1 . . . B N centered on a second optical axis of the modulated light and setting sizes of the second regions B 1 . . . B N such that second integration values of a modulated light intensity distribution in a second section including the second optical axis of the modulated light, the second integration values being obtained by integrating the modulated light intensity distribution in the respective second regions B 1 . . . B N , are equal to each other, obtaining an optical path length L n from the first region A n to the second region B n (n is each integer from 1 to N), and determining a phase of the first region A n based on the optical path length L n . 5. The phase modulation apparatus according to claim 4 , further comprising a measurement section configured to measure the readout light intensity distribution in the first section including the first optical axis of the readout light which enters the phase modulation plane. 6. The phase modulation apparatus according to claim 4 , wherein optical components are not provided on an optical path of the modulated light from the phase modulation plane to the target plane. 7. The phase modulation apparatus according to claim 4 , further comprising an objective lens disposed on the target plane. 8. A phase modulation apparatus comprising: a light source configured to output readout light of which a readout light intensity distribution in a first section including a first optical axis of the readout light is axially symmetric with respect to the first optical axis; a spatial light modulator comprising a phase modulation plane including a plurality of two-dimensionally arrayed modulation regions, and configured to modulate the readout light in phase in each of the plurality of modulation regions to generate modulated light; and a control section configured to control a phase distribution to be displayed on the phase modulation plane such that the modulated light has a predetermined intensity distribution on a target plane separated by a predetermined optical distance from the phase modulation plane, wherein the control section comprises a storage configured to store the phase distribution, and the phase distribution is calculated by dividing a light entering region on the phase modulation plane which the readout light enters into N (N is an integer of 2 or more) concentric first regions A 1 . . . A N centered on the first optical axis of the readout light and setting sizes of the first regions A 1 . . . A N such that first integration values of the readout light intensity distribution in the first section including the first optical axis of the readout light, the first integration values being obtained by integrating the readout light intensity distribution in the respective first regions A 1 . . . A N , are equal to each other, dividing a target region on the target plane into N concentric second regions B 1 . . . B N centered on a second optical axis of the modulated light and setting sizes of the second regions B 1 . . . B N such that second integration values of a modulated light intensity distributio
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