Maize ubiquitin promoters

Patent metadata

Field	Value
Publication number	US-9725730-B2
Application number	US-201414587762-A
Country	US
Kind code	B2
Filing date	Dec 31, 2014
Priority date	Dec 31, 2013
Publication date	Aug 8, 2017
Grant date	Aug 8, 2017

Abstract

The Zea mays c.v. B73 Ubiquitin-1 ( Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

First claim

What is claimed is:

1. A method for purifying a polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2, the method comprising:
a) identifying the polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2;
b) producing a plurality of oligonucleotide primer sequences, wherein the oligonucleotide primer sequences bind to the polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2;
c) amplifying the polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2 from a DNA sample with oligonucleotide primer sequences selected from the plurality of oligonucleotide primer sequences; and,
d) further purifying the amplified polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2.

2. The method of claim 1 , wherein the purified polynucleotide sequence comprising a sequence identity of at least 90% to SEQ ID NO: 2 is operably linked to a transgene.

3. The method of claim 2 , wherein the operably linked transgene encodes a polypeptide or a small RNA.

Assignees

• Dow Agrosciences Llc

Inventors

• Kumar Sandeep
• Gupta Manju
• Wright Terry R
• Jayne Susan M
• Smith Doug A
• Alabed Diaa

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N15/10

  Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) · CPC title

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/8274

  for herbicide resistance · CPC title

• A01H6/4678

  Triticum sp. [wheat] · CPC title