Centrifuge for separating a sample into at least two components

Patent metadata

Field	Value
Publication number	US-9714945-B2
Application number	US-201414201744-A
Country	US
Kind code	B2
Filing date	Mar 7, 2014
Priority date	Dec 7, 2007
Publication date	Jul 25, 2017
Grant date	Jul 25, 2017

Abstract

The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber.

First claim

The invention claimed is:

1. A centrifuge chamber configured to rotate about an axis so as to apply a centrifugal force to a sample of cells inside the chamber and thereby to separate the sample into two or more portions, wherein the chamber comprises:
a base plate, the center of which is substantially perpendicular to the rotational axis of the chamber,
a cladding substantially perpendicular to the base plate,
a cover plate configured to cover the cladding, thereby forming an interior compartment between the base plate and the cover plate that is circumscribed by the cladding;
a sample inlet port; and
one or more openings in the base plate covered by a gas-permeable membrane, the openings being configured for delivery of gasses into the chamber to support culturing of cells;
wherein the centrifuge chamber is configured such that a sample can pass through the chamber by way of the sample inlet port, and air or gas to support culturing of cells in the chamber can pass through the chamber by way of the openings, both without stopping centrifugation of the chamber.

2. A centrifuge chamber configured to rotate about an axis so as to apply a centrifugal force to a sample of cells inside the chamber that is sufficient to separate the sample into two or more different cell types,
wherein the chamber comprises:
a base plate, the center of which is substantially perpendicular to the rotational axis of the chamber,
a cladding substantially perpendicular to the base plate,
a cover plate configured to cover the cladding, thereby forming an interior compartment between the base plate and the cover plate that is circumscribed by the cladding;
a sample inlet port; and
one or more openings for air or gas;
wherein the centrifuge chamber is constructed to withstand a rotational force of 1,000×g when rotated about the axis, and
wherein the centrifuge chamber is configured such that a sample can pass through the chamber by way of the sample inlet port, and air or gas to support culturing of cells in the chamber can pass through the chamber by way of the opening, both without stopping centrifugation of the chamber.

3. The chamber of claim 1 or claim 2 , wherein the inlet port is connected to the interior compartment of the centrifuge chamber by way of a central line that passes along the rotational axis.

4. The chamber of claim 3 , wherein the chamber comprises a rotational bearing through which the central line passes.

5. The chamber of claim 3 , wherein the chamber comprises a rotational seal through which the central line passes.

6. The chamber of claim 1 or claim 2 , comprising an outlet port configured for decanting cells from the sample during centrifugation, wherein both the inlet port and the outlet port are connected to the interior compartment by way of an inner line and an outer line that both pass along the rotational axis.

7. The chamber of claim 2 , wherein the opening for air or gas is located in the base plate.

8. The chamber of claim 1 or claim 2 , comprising a plurality of parallel flat layered surfaces that are perpendicular to the rotational axis and separate and apart from the base plate and the cover plate, wherein the layered surfaces are configured for supporting and culturing cells during centrifugation.

9. The chamber of claim 8 ,
wherein the surfaces are such that cells selectively adhere, thereby configuring the chamber to separate cells during rotation such that cells adhering to the surfaces may be separated from cells with lesser affinity for the surfaces.

10. The chamber of claim 9 , wherein the surfaces are configured such that cells adhering to the surfaces can be disconnected from the surfaces by intermittent rotation or by braking during rotation of the chamber.

11. The chamber of claim 1 or claim 2 , having channels inside the chamber configured for gas flow that are assembled in a spiral pattern adjacent to the base plate.

12. The chamber of claim 1 , constructed to withstand a rotational force of 1,000×g.

Assignees

• Miltenyi Biotec Gmbh

Inventors

• Miltenyi Stefan
• Schimmelpfennig Winfried
• Lantow Holger
• Neuschäfer Elmar Niklas
• Biehl Martin
• Kabaha Eiad
• Schulz Jürgen

Classifications

• C12N2501/998

  Proteins not provided for elsewhere · CPC title

• C12N5/0018

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• B01L3/5021

  Test tubes specially adapted for centrifugation purposes · CPC title

• C12N2500/34

  Sugars · CPC title

• B01D21/262

  by using a centrifuge · CPC title