Hybridization linkers
US-9222082-B2 · Dec 29, 2015 · US
Polynucleotide encoding psicose epimerase and method of producing psicose using the same
US9701953B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9701953-B2 |
| Application number | US-201415101459-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 4, 2014 |
| Priority date | Dec 26, 2013 |
| Publication date | Jul 11, 2017 |
| Grant date | Jul 11, 2017 |
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Abstract
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A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.
First claim
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The invention claimed is: 1. A method for producing psicose, comprising reacting at least one selected from the group consisting of a protein comprising the amino acid sequence of SEQ ID NO: 1, a recombinant cell harboring a recombinant vector carrying a polynucleotide encoding the protein, a culture broth of the recombinant cell, and a lysate of the recombinant cell, with D-fructose. 2. The method of claim 1 , wherein the D-fructose is used at a concentration of 55 to 75% (w/w). 3. The method of claim 1 , wherein the reacting is carried out at a pH of 7 to 10. 4. The method of claim 1 , wherein the reacting is carried out at a temperature of 40 to 70° C. 5. The method of claim 1 , which further comprises adding at least one selected from the group consisting of a copper ion, a manganese ion, a magnesium ion, a zinc ion, a nickel ion, a cobalt ion, an iron ion, an aluminum ion, and a calcium ion. 6. The method of claim 2 , which further comprises adding at least one selected from the group consisting of a copper ion, a manganese ion, a magnesium ion, a zinc ion, a nickel ion, a cobalt ion, an iron ion, an aluminum ion, and a calcium ion. 7. The method of claim 3 , which further comprises adding at least one selected from the group consisting of a copper ion, a manganese ion, a magnesium ion, a zinc ion, a nickel ion, a cobalt ion, an iron ion, an aluminum ion, and a calcium ion. 8. The method of claim 4 , which further comprises adding at least one selected from the group consisting of a copper ion, a manganese ion, a magnesium ion, a zinc ion, a nickel ion, a cobalt ion, an iron ion, an aluminum ion, and a calcium ion. 9. The method of claim 1 , wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO: 2.
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- What does patent US9701953B2 cover?
- A psicose 3-epimerase, a polynucleotide encoding the enzyme, a recombinant vector carrying the polynucleotide, a recombinant cell harboring the recombinant vector, and use thereof are provided.
- Who is the assignee on this patent?
- Samyang Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N9/90. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jul 11 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).