Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US-8945884-B2 · Feb 3, 2015 · US
Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites
US2015225729A1 · US · A1
| Field | Value |
|---|---|
| Publication number | US-2015225729-A1 |
| Application number | US-201414578336-A |
| Country | US |
| Kind code | A1 |
| Filing date | Dec 19, 2014 |
| Priority date | Aug 21, 2000 |
| Publication date | Aug 13, 2015 |
| Grant date | — |
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Abstract
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The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.
First claim
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1 . An isolated nucleic acid molecule comprising: (a) one or more recombination sites; and (b) one or more topoisomerase recognition sites and/or one or more topoisomerases. 2 . The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule is a circular molecule. 3 . The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule comprises two or more recombination sites. 4 . The nucleic acid molecule of c…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US2015225729A1 cover?
- The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are ins…
- Who is the assignee on this patent?
- Life Technologies Corp
- What technology area does this patent fall under?
- Primary CPC classification C12N15/66. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Thu Aug 13 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (A1). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).