Assay method using encoded particle-based platform

Patent metadata

Field	Value
Publication number	US-9689878-B2
Application number	US-201614994664-A
Country	US
Kind code	B2
Filing date	Jan 13, 2016
Priority date	May 17, 2011
Publication date	Jun 27, 2017
Grant date	Jun 27, 2017

Abstract

Provided is an assay method using an encoded particle-based platform. In the assay method, first, a plurality of encoded particles having codes distinguishable from one another according to kinds of included target materials are prepared. The plurality of encoded particles are provided onto a plate including a plurality of wells by pipetting, and disposed in the plurality of wells by a self-assembly method. An analyte is provided into the plurality of wells. The codes of the plurality of encoded particles disposed in the plurality of wells are decoded. The target materials of the plurality of encoded particles are released to cause a reaction between the target materials and the analyte.

First claim

What is claimed is:

1. A method of selecting a target material reacting with an analyte based on an encoded particle-based platform, comprising the steps of:
preparing a plurality of encoded particles including the target material, the plurality of encoded particles having one or more codes distinguishable from one another corresponding to the target material, wherein the codes distinguish the target material;
preparing a first plate including first wells, providing the plurality of encoded particles onto the first plate by pipetting, and disposing the plurality of encoded particles in the first wells;
preparing a second plate including second wells and disposing the analyte in the second wells;
attaching the first plate to the second plate where the first wells of the first plate and the second wells of the second plate correspond to each other;
decoding the codes of the plurality of encoded particles disposed in each of the first wells;
releasing the target material by diffusing from the plurality of encoded particles via the step of attaching the first plate to the second plate, wherein the target material is released into the second wells and a reaction is caused between the target material and the analyte; and
selecting the target material which reacts with the analyte,
wherein the step of preparing the plurality of encoded particles comprises:
mixing the target material and a polymer to form a solution in a resin form; and
solidifying the solution formed in the resin form to form the encoded particles in which the target material is included in the polymer.

2. A method of selecting a target material reacting with an analyte based on an encoded particle-based platform, comprising the steps of:
preparing a plurality of encoded particles including the target material, the plurality of encoded particles having one or more codes distinguishable from one another corresponding to the target material, wherein the codes distinguish the target material;
preparing a first plate including first wells, providing the plurality of encoded particles onto the first plate by pipetting, and disposing the plurality of encoded particles in the first wells;
preparing a second plate including second wells and disposing the analyte in the second wells;
attaching the first plate to the second plate where the first wells of the first plate and the second wells of the second plate correspond to each other;
decoding the codes of the plurality of encoded particles disposed in each of the first wells;
releasing the target material by diffusing from the plurality of encoded particles via the step of attaching the first plate to the second plate, wherein the target material is released into the second wells and a reaction is caused between the target material and the analyte; and
selecting the target material which reacts with the analyte,
wherein the step of preparing the plurality of encoded particles comprises:
dissolving the target material in poly-N-isopropylacrylamide (PNIPAAM);
mixing the PNIPAAM and polyethylene glycol-diacrylate (PEG-DA) to produce a composite in a resin form; and
solidifying the composite.

3. A method of selecting a target material reacting with an analyte based on an encoded particle-based platform, comprising the steps of:
preparing a plurality of encoded particles including the target material, the plurality of encoded particles having one or more codes distinguishable from one another corresponding to the target material, wherein the codes distinguishe the target material;
preparing a first plate including first wells, providing the plurality of encoded particles onto the first plate by pipetting, and disposing the plurality of encoded particles in the first wells;
preparing a second plate including second wells and disposing the analyte in the second wells;
attaching the first plate to the second plate where the first wells of the first plate and the second wells of the second plate correspond to each other;
decoding the codes of the plurality of encoded particles disposed in each of the first wells;
releasing the target material by diffusing from the plurality of encoded particles via the step of attaching the first plate to the second plate, wherein the target material is released into the second wells and a reaction is caused between the target material and the analyte; and
selecting the target material which reacts with the analyte,
wherein the step of preparing the plurality of encoded particles comprises:
forming a first polymer layer;
forming a target material layer on the first polymer layer; and
forming a second polymer layer sealing the target material layer.

4. The method of claim 3 , wherein the step of forming the first polymer layer includes providing and solidifying a fluidic polymer, and the step of forming the second polymer layer includes providing and solidifying the fluidic polymer.

5. The method of claim 3 , wherein the step of forming the target material layer includes providing a fluidic polymer including the target material, and solidifying the fluidic polymer.

6. The method of claim 5 , further comprising:
mixing the target material and a polymer to form a solution in a resin form on the first polymer layer; and
solidifying the solution formed in the resin form.

7. The method of claim 3 , wherein the step of forming the target material layer includes:
forming a hydrophilic polymer layer on the first polymer layer; and
causing the hydrophilic polymer layer to absorb the target material.

8. The method of claim 3 , wherein the step of forming the target material layer includes:
forming a first structure in a form of a wall on the first polymer layer along an edge of the first polymer layer using a hydrophobic polymer;
forming a second structure on a sidewall in the first structure using a hydrophilic polymer; and
forming the target material layer in the second structure on the first polymer layer.

Assignees

• Snu R&Db Foundation
• Quantamatrix Inc

Inventors

• Kwon Sung Hoon
• Chung Su Eun
• Lee Sung Hoon
• Park Wook
• Song Young Hoon

Classifications

• G01N33/585 Primary

  with a particulate label, e.g. coloured latex · CPC title

• Y10T436/143333

  Saccharide [e.g., DNA, etc.] · CPC title

• G01N33/54313 Primary

  the carrier being characterised by its particulate form · CPC title

• G01N33/58

  involving labelled substances (G01N33/53 takes precedence) · CPC title

• G01N35/10

  Devices for transferring samples {or any liquids} to, in, or from, the analysis apparatus, e.g. suction devices, injection devices {(G01N35/0099 takes precedence)} · CPC title