Blue-colored gold nanoparticles for immunological measurement, process for production of same, and measurement method using same

Patent metadata

Field	Value
Publication number	US-9360431-B2
Application number	US-201113883213-A
Country	US
Kind code	B2
Filing date	Nov 4, 2011
Priority date	Nov 5, 2010
Publication date	Jun 7, 2016
Grant date	Jun 7, 2016

Abstract

Gold nanoparticles which comprises organic buffer containing a piperazine ring, gold, and an organic acid having reducing properties and which shows a blue color by visually view when it is dispersed as a colloidal solution, can be produced easily by conducting a nucleus formation step by reacting organic acid containing a piperazine ring with a solution of a first gold salt to form nucleus gold nanoparticles and a growth step by simultaneously adding and reacting a solution of a second gold salt and an organic acid having reducing properties with a solution of the nucleus gold nanoparticle to grow the nucleus gold nanoparticles. The produced gold nanoparticles can be used as labeling particles in an immunological measurement method.

First claim

The invention claimed is:

1. A plurality of blue-colored gold nanoparticles having an average particle nucleus size of from 20 to 60 nm, an average particle size of from 60 to 120 nm, four or more protrusions per nucleus, and a protrusion length of from 5 to 50 nm.

2. The blue-colored gold nanoparticles according to claim 1 , wherein the maximum absorption wavelength in ultraviolet visible absorption spectra falls within a range of from 570 to 800 nm.

3. The blue-colored gold nanoparticles according to claim 1 , wherein the gold nanoparticles are graft-shaped particles, multipod-shaped particles, or confeito-shaped particles having a three-dimensional protrusion.

4. The blue-colored gold nanoparticles according to claim 1 , obtained by growing the periphery of the nucleus composed of gold nanoparticles.

5. A colloidal solution of blue-colored gold nanoparticles, comprising the blue-colored gold nanoparticles as described in claim 1 ; organic acid containing a piperazine ring which is a Good's buffer component; and an organic acid having reducing properties and is dispersed as a colloidal solution.

6. A labeling substance for immunological measurement comprising at least two kinds of blue-colored gold nanoparticles as described in claim 1 that differ in shape.

7. The labeling substance for immunological measurement according to claim 6 , which comprises at least two kinds of gold nanoparticles of different shapes which are spherical gold nanoparticles and graft-shaped, multipod-shaped, or confeito-shaped gold nanoparticles having a three-dimensional protrusion.

8. An immunological measurement method using the blue-colored gold nanoparticles as described in claim 1 as a labeling substance comprising the steps of: combining an antibody-bound to one or more of the plurality of blue colored nanoparticles according to claim 1 with a sample containing a detection object, wherein the antibody binds specifically to the detection object to form a complex comprising the detection object and the antibody-bound one or more of the plurality of blue colored nanoparticles according claim 1 ; measuring a luminescent signal from the complex.

9. A method for producing blue-colored gold nanoparticles having an average particle size of 60 to 120 nm, comprising a nucleus formation step by reacting organic acid containing a piperazine ring which is a Good's buffer component with a solution of a first gold salt to form nucleus gold nanoparticles and a growth step by simultaneously adding and reacting a solution of a second gold salt and an organic acid having reducing properties with a solution of the nucleus gold nanoparticle to grow the nucleus gold nanoparticles.

10. The method for producing blue-colored gold nanoparticles according to claim 9 , wherein the growth step is conducted at a reaction temperature of 10° C. or greater and less than 40° C.

11. The method for producing blue-colored gold nanoparticles according to claim 9 , wherein the organic acid in the growth step has a concentration of from 0.075 to 0.15 mM.

12. The method for producing blue-colored gold nanoparticles according to claim 11 , wherein the organic acid containing piperazine ring which is a Good's buffer component is one or more organic acids selected from the group consisting of 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-hydroxyethyl)piperazine-1-(2-hydroxypropane-3-sulfonic acid), piperazine-1,4-bis(2-ethanesulfonic acid), 3-[4-(2-hydroxyethyl)-1-piperazinyl]propanesulfonic acid, and piperazine-1,4-bis(2-hydroxy-3-propanesulfonic acid).

13. The method for producing blue-colored gold nanoparticles according to claim 9 , wherein the organic acid having reducing properties is one or more organic acids selected from the group consisting of tartaric acid, tartrates, tannic acid, tannates, ascorbic acid, ascorbates, citric acid, and citrates.

14. The method for producing blue-colored gold nanoparticles according to claim 9 , wherein in the growth step, the organic acid containing a piperazine ring which is a Good's buffer component is used in combination with the organic acid having reducing properties.

Assignees

• Kato Yuya
• Ito Daisuke
• Kitani Yoshiko
• Tanaka Precious Metal Ind

Inventors

• Kato Yuya
• Ito Daisuke
• Kitani Yoshiko

Classifications

• B22F1/0547 Primary

  Nanofibres or nanotubes · CPC title

• B22F2304/056

  Particle size above 100 nm up to 300 nm · CPC title

• B22F9/24

  starting from liquid metal compounds, e.g. solutions · CPC title

• B82Y30/00

  Nanotechnology for materials or surface science, e.g. nanocomposites · CPC title

• B22F2301/255

  Silver or gold · CPC title