Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
US-9211315-B2 · Dec 15, 2015 · US
US9688973B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9688973-B2 |
| Application number | US-201314104756-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 12, 2013 |
| Priority date | Jun 29, 2004 |
| Publication date | Jun 27, 2017 |
| Grant date | Jun 27, 2017 |
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The present invention relates to isolated polypeptides having alpha-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
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What is claimed is: 1. A nucleic acid construct comprising a gene encoding a protein operably linked to a nucleotide sequence encoding a signal peptide selected from the group consisting of: nucleotides 1 to 42 of SEQ ID NO: 1, nucleotides 1 to 145 of SEQ ID NO: 3, and nucleotides 1 to 57 of SEQ ID NO: 5, wherein the gene is heterologous to the nucleotide sequence encoding the signal peptide. 2. A recombinant expression vector comprising the nucleic acid construct of claim 1 . 3. A recombinant host cell comprising the nucleic acid construct of claim 1 . 4. A method for producing a protein comprising (a) cultivating the recombinant host cell of claim 3 under conditions conducive for production of the protein; and (b) recovering the protein.
Amylases · CPC title
involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis · CPC title
acting on alpha -1,4-glucosidic bonds · CPC title
Preparation or treatment of the mash · CPC title
Enzymes · CPC title
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