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US-9217140-B2 · Dec 22, 2015 · US
Method for enhancing extracellular secretion of recombinant proteins in Escherichia coli by co-expressing Thermobifida fusca cutinase
US9109212B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9109212-B2 |
| Application number | US-201313935519-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 4, 2013 |
| Priority date | Jul 5, 2012 |
| Publication date | Aug 18, 2015 |
| Grant date | Aug 18, 2015 |
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Abstract
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The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli . Increased extracellular secretion of target proteins can shorten cell culture time, reduce the formation of inclusion bodies and increase production of target proteins.
First claim
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What is claimed is: 1. A method for secretory expression of T. fusca cutinase in E. coli without mediation of a signal peptide, comprising cloning a mature cutinase gene from T. fusca into an expression vector that does not comprise a signal peptide, expressing said mature cutinase in an E. coli host strain and obtaining said T. fusca cutinase from the culture supernatant. 2. The method of claim 1 , comprising the steps of: 1) Amplify a gene encoding…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N9/20Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US9109212B2 cover?
- The present invention provides a method of increasing extracellular secretion of secretory proteins by co-expressing the secretory proteins with a mature cutinase. Cutinase can improve the permeability of E. coli cell membrane without destroying the membrane, and thus facilitate cross-membrane transfer of the secretory proteins co-expressed in E. coli . Increased extracellular secretion of t…
- Who is the assignee on this patent?
- Univ Jiangnan
- What technology area does this patent fall under?
- Primary CPC classification C12N9/20. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 18 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).