Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions

Patent metadata

Field	Value
Publication number	US-9670451-B2
Application number	US-201414548617-A
Country	US
Kind code	B2
Filing date	Nov 20, 2014
Priority date	Oct 8, 2010
Publication date	Jun 6, 2017
Grant date	Jun 6, 2017

Abstract

Embodiments described herein generally relate to methods and systems for using an air removal chamber as a control for a process in a cell expansion system. The air removal chamber may be mounted on a fluid conveyance assembly for use with the system. Fluid is pumped into a fluid containment chamber of the air removal chamber, in which the level of fluid in the fluid containment chamber may be monitored through the use of one or more sensors. The sensors are capable of detecting air, a lack of fluid, fluid, and/or a gas/fluid interface, e.g., an air/fluid interface, at measuring positions within the air removal chamber. Protocols for use with the system may include one or more stop conditions. In an embodiment, the stopping of a process is automated based on the detection of air, a lack of fluid, and/or a gas/fluid interface in the air removal chamber.

First claim

What is claimed is:

1. A closed cell expansion system comprising a capability to control a process of a protocol, the closed cell expansion system comprising:
a fluid conveyance assembly, wherein the fluid conveyance assembly comprises a bioreactor;
an air removal chamber, the air removal chamber comprising a fluid containment chamber, wherein the fluid containment chamber comprises:
a fluid entrance aperture;
a fluid exit aperture; and
a vent aperture;

at least one sensor for detecting a gas/fluid interface in the fluid containment chamber; and
a controller, in communication with the at least one sensor, wherein the controller is operable to:
signal to start a first process of a first protocol, wherein the first protocol comprises loading cells into the bioreactor, wherein the first protocol comprises a first process and a second process, wherein the first process comprises loading the cells into the closed cell expansion system, and wherein a first stop condition for the first process involves the air removal chamber, the signal to start the first process comprising:
signal to start one or more pumps for pumping a fluid comprising the cells through the fluid entrance aperture and into the fluid containment chamber of the air removal chamber;

receive an indication from the at least one sensor when the at least one sensor detects the gas/fluid interface;
in response to receiving the indication from the at least one sensor, determine if the first stop condition for the first process is met;
when the first stop condition for the first process is met, signal to stop the first process, the signal to stop the first process comprising signal to stop the one or more pumps to stop the first process; and
signal to start the second process of the first protocol, wherein the second process comprises chasing the cells from the air removal chamber into a circulation loop of the closed cell expansion system, wherein a second stop condition for the second process involves the air removal chamber, and wherein the second stop condition is different from the first stop condition.

2. The closed cell expansion system as defined in claim 1 , wherein the at least one sensor comprises an ultrasonic sensor.

3. The closed cell expansion system as defined in claim 1 , wherein the first protocol comprises a third process, and wherein the third process comprises chasing the cells from the circulation loop into the bioreactor.

4. The closed cell expansion system as defined in claim 1 , wherein the circulation loop comprises an intracapillary loop.

5. The closed cell expansion system as defined in claim 3 , wherein the circulation loop comprises an intracapillary loop.

6. The closed cell expansion system as defined in claim 1 , wherein the first protocol comprises loading cells into the bioreactor with circulation.

7. The closed cell expansion system as defined in claim 1 , wherein the at least one sensor comprises an optical sensor.

8. The closed cell expansion system as defined in claim 1 , wherein the controller, in communication with the at least one sensor, is further operable to:
signal to start a first process of a second protocol, wherein the second protocol is different from the first protocol.

Assignees

• Terumo Bct Inc

Inventors

• Dilorenzo Thomas G
• Stanton Iv Edward Allan
• Antwiler Glen Delbert
• Kinzie Michael E
• Nankervis Brian J
• Givens Monique
• Medina Casey V
• Dodd Jon A

Classifications

• C12M29/20

  Degassing; Venting; Bubble traps (means for collection or storage of gas C12M23/36; gas collection apparatus for laboratory use B01L5/02) · CPC title

• G06F8/38

  for implementing user interfaces · CPC title

• C12M25/12

  the culture medium flowing outside the fiber or tube · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• C12M41/44 Primary

  of volume or liquid level · CPC title