Premounted fluid conveyance assembly for cell expansion system and method of use associated therewith

Patent metadata

Field	Value
Publication number	US-9284523-B2
Application number	US-60606409-A
Country	US
Kind code	B2
Filing date	Oct 26, 2009
Priority date	Oct 27, 2008
Publication date	Mar 15, 2016
Grant date	Mar 15, 2016

Abstract

A premounted fluid conveyance assembly for use with a cell expansion machine comprises a tubing-organizer and a fluid conveyance system. The fluid conveyance system is at least partially attached to the tubing-organizer, and the fluid conveyance system comprises an oxygenator or gas transfer module, a length of tubing and a bioreactor all fluidly interconnected. The premounted fluid conveyance assembly is adapted to be detachably-attached to the cell expansion machine. Accordingly, after a premounted fluid conveyance assembly is used, it is removed from the cell expansion machine by disengaging it from the cell expansion machine. Thereafter, another premounted fluid conveyance assembly can be attached in place of the previously used premounted fluid conveyance assembly.

First claim

What is claimed is:

1. A premounted fluid conveyance assembly for use with a cell expansion machine, the premounted fluid conveyance assembly comprising:
a tubing-organizer comprising an oblong shaped access aperture that is shaped to mate with a fitting on a rotatable shaft of the cell expansion machine, wherein the tubing-organizer further comprises a plurality of sensor ports to provide access between pressure and temperature sensors and sensor couplings;
a fluid conveyance system at least partially attached to the tubing-organizer, the fluid conveyance system comprising:
a gas transfer module;
a length of tubing fluidly associated with the gas transfer module;
a rotatable hollow fiber bioreactor fluidly associated with the length of tubing and the gas transfer module; and
a bioreactor coupling comprising a shaft fitting, wherein the bioreactor coupling is coupled to the rotatable hollow fiber bioreactor and the shaft fitting comprises at least a spring member for engaging with the rotatable shaft of the cell expansion machine to rotate the rotatable hollow fiber bioreactor;
a plurality of pump tubing conduits, at least a portion of each of the plurality of pump tubing conduits extending beyond a support member and looping back to a perimeter of the support member, wherein each of the plurality of pump tubing conduits is engageable with at least one of an IC circulation pump, an EC circulation pump, an IC inlet pump, or an IC inlet pump on the cell expansion machine; and
wherein the tubing-organizer and the fluid conveyance system are adapted to be detachably-attached to the cell expansion machine.

2. The premounted fluid conveyance assembly of claim 1 , wherein the tubing-organizer comprises a plurality of apertures and a plurality of tubing holders spaced across a height and a width of a wall and a perimeter of the tubing-organizer.

3. The premounted fluid conveyance assembly of claim 1 , wherein the fluid conveyance system further comprises an air removal chamber.

4. A premounted fluid conveyance assembly for use with a cell expansion machine, the cell expansion machine including at least one valve clamp and a rotatable shaft, the premounted fluid conveyance assembly comprising:
a fluid conveyance system comprising:
a gas transfer module;
a length of tubing fluidly associated with the gas transfer module;
a rotatable hollow fiber bioreactor fluidly associated with the length of tubing and the gas transfer module;
a bioreactor coupling comprising a shaft fitting, wherein the bioreactor coupling is coupled to the rotatable hollow fiber bioreactor and the shaft fitting comprises at least a spring member for engaging with the rotatable shaft of the cell expansion machine to rotate the rotatable hollow fiber bioreactor;
a support member including a plurality of holding elements, the plurality of holding elements including a gas transfer module mounting post and a plurality of tubing guides holding at least a portion of the length of tubing, wherein the support member further comprises a shaft access aperture that is oblong shaped to mate with a fitting on the rotatable shaft of the cell expansion machine and the support member further comprising a plurality of sensor ports to provide access between pressure and temperature sensors and sensor couplings;
a plurality of pump tubing conduits, at least a portion of each of the plurality of pump tubing conduits extending beyond the support member and looping back to a perimeter of the support member, wherein each of the plurality of pump tubing conduits is engageable with at least one of an IC circulation pump, an EC circulation pump, an IC inlet pump, or an IC inlet pump on a cell expansion machine; and
wherein the support member and the fluid conveyance system are adapted to be detachably-attached to the cell expansion machine.

5. The premounted fluid conveyance assembly of claim 4 , wherein the support member comprises a valve access opening adapted for allowing contact of the at least one valve clamp with a portion of the length of tubing.

6. The premounted fluid conveyance assembly of claim 4 , wherein the fluid conveyance system further comprises an air removal chamber.

7. A premounted fluid conveyance assembly for use with a cell expansion machine, the cell expansion machine including at least one valve clamp, at least one pump, and a rotatable shaft associated within a shaped fitting, the premounted assembly comprising:
a fluid conveyance system comprising:
a gas transfer module;
a length of tubing fluidly associated with the gas transfer module;
a rotatable hollow fiber bioreactor fluidly associated with the length of tubing and the gas transfer module;
a bioreactor coupling comprising a shaft fitting, wherein the bioreactor coupling is coupled to the rotatable hollow fiber bioreactor and the shaft fitting comprises a spring member for engaging with the rotatable shaft of the cell expansion machine to rotate the rotatable hollow fiber bioreactor;

a means for supporting at least a portion of the fluid conveyance system, wherein the means for supporting comprises:
a shaped access aperture that is oblong shaped to mate with a fitting on the rotatable shaft of the cell expansion machine;
a plurality of sensor ports to provide access between pressure and temperature sensors and sensor couplings;
a means for detachably-attaching the means for supporting to the cell expansion machine;
a means for holding the gas transfer module; and
a means for holding the length of tubing fluidly associated with the gas transfer module; and
a plurality of pump tubing conduits, at least a portion of each of the plurality of pump tubing conduits extending beyond the means for supporting and looping back to a perimeter of the means for supporting, wherein each of the plurality of pump tubing conduits is engageable with at least one of an IC circulation pump, an EC circulation pump, an IC inlet pump, or an IC inlet pump on the cell expansion machine.

8. The premounted fluid conveyance assembly of claim 7 , wherein the means for supporting comprises a tubing-organizer.

9. The premounted fluid conveyance assembly of claim 7 , wherein the means for holding the length of tubing fluidly associated with the gas transfer module comprises a plurality of tubing guides.

10. The premounted fluid conveyance assembly of claim 7 , wherein the means for supporting includes a plurality of apertures spaced across a height and a width of a wall and a perimeter of the means for supporting.

11. The premounted fluid conveyance assembly of claim 7 , further comprising a means for guiding a portion of the length of tubing between a back portion and a door of the cell expansion machine.

12. The premounted fluid conveyance assembly of claim 11 , wherein the means for guiding comprises a plurality of tubing guide slots.

13. The premounted fluid conveyance assembly of claim 7 , wherein the fluid conveyance system further comprises an air removal chamber.

Assignees

• Dodd Jon A
• Dilorenzo Thomas G
• Corbin Iii Frank
• Antwiler Glen Delbert
• Terumo Bct Inc

Inventors

• Dodd Jon A
• Dilorenzo Thomas G
• Corbin Iii Frank
• Antwiler Glen Delbert

Classifications

• C12M23/48 Primary

  Holding appliances; Racks; Supports (holding devices for laboratory apparatus B01L9/00) · CPC title

• C12M25/10

  Hollow fibers or tubes (hollow fiber modules in general B01D63/02) · CPC title

• C12M25/12

  the culture medium flowing outside the fiber or tube · CPC title

• C12M23/42

  Integrated assemblies, e.g. cassettes or cartridges · CPC title

• C12M29/00

  Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B) · CPC title