Methods and Compositions for Generating Bioactive Assemblies of Increased Complexity and Uses
US-2015374846-A1 · Dec 31, 2015 · US
RNase H-based assays utilizing modified RNA monomers
US9644198B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9644198-B2 |
| Application number | US-201313959637-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 5, 2013 |
| Priority date | Apr 30, 2008 |
| Publication date | May 9, 2017 |
| Grant date | May 9, 2017 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
First claim
Opening claim text (preview).
What is claimed is: 1. A hot start enzyme composition comprising an RNase H enzyme and a chemical modification to said RNase H enzyme, wherein said chemical modification reversibly inactivates said RNase H enzyme, and wherein said chemical modification is formed by reacting said RNase H enzyme with a maleic acid anhydride analog. 2. The hot start enzyme composition of claim 1 , wherein said RNase H enzyme is Pyrococcus abyssi RNase H enzyme. 3. The hot start enzyme composition of claim 2 , wherein said Pyrococcus abyssi RNase H enzyme comprises the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4. 4. The hot start enzyme composition of claim 1 , wherein said maleic anhydride analog is citraconic anhydride. 5. A hot start enzyme composition comprising an RNase H enzyme and an antibody bound to said RNase H enzyme, wherein said antibody reversibly inactivates said RNase H enzyme when said antibody is bound to said RNase H enzyme. 6. The hot start enzyme composition of claim 5 , wherein said RNase H enzyme is Pyrococcus abyssi RNase H enzyme. 7. The hot start enzyme composition of claim 6 , wherein said Pyrococcus abyssi RNaseH enzyme comprises the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 4. 8. The hot start enzyme composition of claim 6 , wherein said antibody is an antibody that specifically binds said Pyrococcus abyssi RNase H enzyme.
Assignees
Inventors
Classifications
- C12N9/96Primary
Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates · CPC title
using modified primers or templates · CPC title
Nucleic acid amplification reactions · CPC title
Hot start · CPC title
Endonuclease · CPC title
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Frequently asked questions
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- What does patent US9644198B2 cover?
- The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing…
- Who is the assignee on this patent?
- Walder Joseph Alan, Behlke Mark Aaron, Rose Scott, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/96. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 09 2017 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).