Production method of polyurethane porous membrane to be used for at least one of applications of cell culture and cancer cell growth inhibition

Patent metadata

Field	Value
Publication number	US-9637722-B2
Application number	US-201414320914-A
Country	US
Kind code	B2
Filing date	Jul 1, 2014
Priority date	Jul 9, 2013
Publication date	May 2, 2017
Grant date	May 2, 2017

Abstract

A polyurethane porous membrane is produced by a simple method to be used for at least one of applications of cell culture and cancer cell growth inhibition. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition comprises: a first step of forming a layer of a polyurethane material which is uncured, on a substrate; and a second step of supplying water vapor to an exposed surface of the layer of the polyurethane material formed on the substrate, which is away from the substrate, so as to cure the polyurethane material and provide the layer of the polyurethane material with a porous structure having a plurality of irregularities on the exposed surface.

First claim

The invention claimed is:

1. A production method of a polyurethane porous membrane to be used for at least one of applications of cell culture and cancer cell growth inhibition, the production method of the polyurethane porous membrane comprising:
a first step of forming a layer of uncured polyurethane material on a substrate, the layer of uncured polyurethane material having a first surface adjacent the substrate and an exposed surface opposite the first surface; and
a second step of supplying water vapor and contacting the exposed surface of the layer of uncured polyurethane material formed on the substrate with an effective amount of the water vapor effective to cure the uncured polyurethane material and to convert the uncured polyurethane material into a polyurethane porous membrane with a porous structure having a thickness of 0.1 to 100 μm and including a plurality of pores open to the exposed surface having an average pore diameter of 0.1 to 100 μm, the plurality of pores open to the exposed surface forming a plurality of irregularities on the exposed surface, wherein
the porous structure of the polyurethane porous membrane only contains a single pore extending along a membrane thickness direction of the polyurethane porous membrane.

2. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 1 ,
wherein the second step includes a reaction temperature and a reaction time effective for forming the polyurethane porous membrane having the thickness of 0.1 to 100 μm.

3. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 1 ,
wherein the second step includes a reaction time effective for forming the polyurethane porous membrane having the thickness of 0.1 to 100 μm.

4. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 1 ,
wherein the plurality of pores open to the exposed surface of the polyurethane porous membrane include pores passing through to the first surface of the layer of polyurethane material adjacent to the substrate.

5. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 4 ,
wherein the second step includes a reaction temperature and a reaction time effective for forming the pores open to the exposed surface of the polyurethane porous membrane and passing through polyurethane porous membrane.

6. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 4 ,
wherein the first step comprises substeps of:
a mixing step of mixing the polyurethane material including at east a polyol and an isocyanate; and
a layer formation step of forming the polyurethane material mixed in the mixing step to the layer, and

wherein an amount of time between the mixing step and the layer formation step and an environment temperature of the polyurethane material between the mixing step and the layer formation step are effective for forming the pores passing through to the first surface of the layer of the polyurethane material adjacent to the substrate.

7. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition according to claim 1 , the production method of the polyurethane porous membrane further comprising:
an additional step of curing the polyurethane material without supplying the water vapor, after the second step.

8. The production method of the polyurethane porous membrane according to claim 1 ,
wherein the substrate is a film made of a material selected from polypropylene, fluororesin, polyethylene terephthalate, polyethylene, polyvinylidene chloride, polyamide and polyimide.

9. The production method of the polyurethane porous membrane according to claim 1 , the production method of the polyurethane porous membrane further comprising:
a fourth step of removing the substrate from the polyurethane porous membrane, after the second step.

10. The production method of the polyurethane porous membrane according to claim 1 , wherein
each of the pores open to the exposed surface of the polyurethane porous membrane has a curved inner wall surface that is convex toward the first surface.

11. The production method of the polyurethane porous membrane according to claim 1 , wherein
the irregularities on the exposed surface of the polyurethane porous membrane have widths of not greater than 20% of the membrane thickness.

12. The production method of the polyurethane porous membrane according to claim 1 , wherein
the second step is carried out in an airtight container, the layer of uncured polyurethane material is arranged in the container with the exposed surface facing downward and the water vapor is supplied from below the layer of uncured polyurethane material.

Assignees

• Toyoda Gosei Kk
• Nat Univ Corp Yamagata Univ

Inventors

• Taki Seitaro
• Mizuno Hisashi
• Nakagawa Hiroyuki
• Hagiyama Toshiyuki
• Yoshimura Atsuki
• Tanaka Masaru
• Sasaki Ayano
• Takahashi Toshifumi
• Ohta Tsuyoshi

Classifications

• A61P35/00

  Antineoplastic agents · CPC title

• C08J9/08

  developing carbon dioxide · CPC title

• C08J2203/10

  Water or water-releasing compounds · CPC title

• C08J2203/02

  CO2-releasing, e.g. NaHCO3 and citric acid · CPC title

• C08J9/125

  Water, e.g. hydrated salts · CPC title