Method for shaping tissue matrices

Patent metadata

Field	Value
Publication number	US-9636435-B2
Application number	US-201113177720-A
Country	US
Kind code	B2
Filing date	Jul 7, 2011
Priority date	Jul 8, 2010
Publication date	May 2, 2017
Grant date	May 2, 2017

Abstract

Methods for shaping tissue matrices are provided. The methods can be used to produce shaped tissue products that retain desired biologic properties without using chemical crosslinking agents.

First claim

What is claimed is:

1. A method for shaping a tissue matrix, comprising:
selecting a collagen-containing tissue matrix;
partially dehydrating the tissue matrix;
applying mechanical forces to the tissue matrix to change the orientation of collagen fibers within the tissue matrix; and
exposing the tissue matrix while partially dehydrated to ionizing radiation to cross-link at least a portion of the tissue matrix.

2. The method of claim 1 , wherein the tissue matrix is an a cellular tissue matrix.

3. The method of claim 1 , wherein the tissue matrix comprises a dermal tissue matrix.

4. The method of claim 1 , wherein the tissue is selected from fascia, pericardial tissue, dura, umbilical cord tissue, placental tissue, cardiac valve tissue, ligament tissue, tendon tissue, arterial tissue, venous tissue, neural connective tissue, urinary bladder tissue, ureter tissue, and intestinal tissue.

5. The method of claim 1 , wherein partially dehydrating the tissue matrix includes removing water to produce a tissue matrix containing between 95% (w/w) and 50% (w/w) water content.

6. The method of claim 5 , wherein the water content is between 80% (w/w) and 65% (w/w).

7. The method of claim 1 , further comprising rehydrating the tissue matrix.

8. The method of claim 1 , wherein the radiation is applied at a dose between 5 Gy and 50 kGy.

9. The method of claim 1 , wherein the radiation is applied at a dose between 5 Gy and 20 kGy.

10. The method of claim 1 , wherein the radiation is applied at a dose of less than 10 kGy.

11. The method of claim 1 , wherein the radiation is applied at a dose of less than 5 kGy.

12. The method of claim 1 , wherein the radiation is applied at a dose of less than 1 kGy.

13. The method of claim 1 , wherein the radiation is selected from gamma radiation, e-beam radiation, and X-ray radiation.

14. The method of claim 1 or 2 , wherein selecting a tissue matrix includes selecting a tissue matrix in the form of a flexible sheet.

Assignees

• Sun Wendell
• Monteiro Gary
• Lifecell Corp

Inventors

• Sun Wendell
• Monteiro Gary

Classifications

• A61F2/12

  Mammary prostheses · CPC title

• A61F2/186

  Nose parts · CPC title

• A61L27/3691 Primary

  characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation · CPC title

• A61L27/24 Primary

  Collagen · CPC title

• A61L2430/04

  for mammary reconstruction · CPC title