Flow cytometry analysis of materials adsorbed to metal salts

Patent metadata

Field	Value
Publication number	US-9618508-B2
Application number	US-201113994536-A
Country	US
Kind code	B2
Filing date	Dec 14, 2011
Priority date	Dec 14, 2010
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

The invention utilizes the techniques and instruments which are typically used in flow cytometry (FC) as a tool for analyzing adsorbed materials. Thus the invention provides a method for analyzing a component which is adsorbed to an insoluble metal salt, comprising steps of: (i) labelling the adsorbed component with a binding reagent; and (ii) analyzing the labelled adsorbed component by flow cytometry.

First claim

The invention claimed is:

1. A flow cytometric method for measuring the amount of a component adsorbed to an insoluble metal salt, wherein the measuring comprises the steps of: (i) labelling the adsorbed component with a labelled binding reagent to produce a labelled component; and (ii) measuring the amount of the labelled adsorbed component on the insoluble metal salt particles by flow cytometry.

2. The method according to claim 1 further comprising, before analysis, the step of adsorbing the component to the insoluble metal salt.

3. The method according to claim 1 , wherein the labelling step is preceded by a blocking step.

4. The method according to claim 1 , wherein the component is a polypeptide antigen.

5. The method according to claim 1 , wherein the component is an immunopotentiator.

6. The method according to claim 1 , wherein the metal salt is an aluminium salt.

7. The method according to claim 6 , wherein the aluminium salt comprises an aluminium hydroxide and/or an aluminium phosphate.

8. The method according to claim 1 , wherein the labelled binding reagent comprises an antibody.

9. The method according to claim 8 , wherein the labelled binding reagent comprises a primary antibody and a secondary antibody.

10. The method according to claim 1 , wherein the labelled binding reagent comprises a fluorophore.

11. A method for analysing the distribution of a component which is adsorbed to an insoluble metal salt particles, comprising steps of: (i) labelling the adsorbed component with a labelled binding reagent to produce a labelled adsorbed component; and (ii) analysing the distribution of the labelled adsorbed component on the insoluble metal salt particles by flow cytometry.

12. The method according to claim 11 further comprising, before analysis, the step of adsorbing the component to the insoluble metal salt particles.

13. The method according to claim 11 , wherein the labelling step is preceded by a blocking step.

14. The method according to claim 11 , wherein the component is a polypeptide antigen.

15. The method according to claim 11 , wherein the component is an immunopotentiator.

16. The method according to claim 11 , wherein the metal salt particles are aluminium metal salt particles.

17. The method according to claim 16 , wherein the aluminium metal salt particles comprises aluminium hydroxide particles and/or an aluminium phosphate particles.

18. The method according to claim 17 , wherein the labelled binding reagent comprises antibody.

19. The method according to claim 18 , wherein the labelled binding reagent comprises a primary antibody and a secondary antibody.

20. The method according to claim 11 , wherein the labelled binding reagent comprises a fluorophore.

Assignees

• Skibinski David
• Laera Donatello
• Nuti Sandra
• Ugozzoli Mildred
• Glaxosmithkline Biologicals Sa

Inventors

• Skibinski David
• Laera Donatello
• Nuti Sandra
• Ugozzoli Mildred

Classifications

• G01N33/537 Primary

  with separation of immune complex from unbound antigen or antibody · CPC title

• G01N33/56911 Primary

  Bacteria · CPC title

• G01N33/68

  involving proteins, peptides or amino acids {(involving lipoproteins G01N33/92)} · CPC title

• G01N33/84

  involving inorganic compounds or pH · CPC title