Alcoholic fermentation yeast and method for producing ethanol using same

Patent metadata

Field	Value
Publication number	US-9617565-B2
Application number	US-201414539382-A
Country	US
Kind code	B2
Filing date	Nov 12, 2014
Priority date	Aug 4, 2010
Publication date	Apr 11, 2017
Grant date	Apr 11, 2017

Abstract

[Problem] To provide an alcoholic fermentation yeast having resistance to limonene, which is a fermentation inhibitor, and a method for producing ethanol using the alcoholic fermentation yeast. [Solution] An alcoholic fermentation yeast Saccharomyces cerevisiae (Deposition No. NITE BP-890), which has resistance to limonene and can grow in the presence of limonene at a concentration of 0.1-0.5 wt %, and a method for producing ethanol using the alcoholic fermentation yeast.

First claim

The invention claimed is:

1. A method of producing ethanol comprising:
squeezing a citrus to produce a juice residue;
mixing the juice residue with slaked lime;
dewatering the juice residue mixed with the slaked lime by pressing the juice residue to produce a juice residue extract; and
(1) mixing the juice residue extract with nitric acid to form an acid-mixed solution having a pH of 3.0 to 4.0 without subjecting the juice residue extract to a three-phase centrifugation, and then adding Saccharomyces Cerevisiae as an alcoholic fermentation yeast having a limonene resistance to the acid-mixed solution to perform alcoholic fermentation and to produce the ethanol, or
(2) subjecting the juice residue extract to a three-phase centrifugation to form a heavy liquid, a light liquid and a solid, then mixing the heavy liquid with nitric acid to form an acid-mixed solution having a pH of 3.0 to 4.0, and adding Saccharomyces Cerevisiae as an alcoholic fermentation yeast having a limonene resistance to the acid-mixed solution to perform alcoholic fermentation and to produce the ethanol.

2. The method according to claim 1 , wherein the alcoholic fermentation is a continuous fermentation performed at a dilution ratio of from 0.04 to 0.08 per hour.

3. The method according to claim 2 , wherein the alcoholic fermentation yeast is Saccharomyces Cerevisiae (Deposition No. NITE BP-890).

4. The method according to claim 1 , wherein the heavy liquid in the three-phase centrifugation comprises limonene having a concentration of 0.01 wt % or lower.

5. The method according to claim 4 , wherein the centrifuge is a disk-type centrifuge.

6. The method according to claim 5 , wherein a centrifugal force of the disk-type centrifuge is 5000 G or larger.

Assignees

• Nippon Steel & Sumikin Eng Co
• Ehime Prefecture

Inventors

• Maekawa Natsuki
• Katoh Yasuhiko
• Kiuchi Takafumi
• Suthasinee Praneetrattananon
• Okamoto Shinji
• Hirayama Kazuko
• Miyaoka Syunsuke
• Tadanobu Hideaki
• Sudo Masahiko

Classifications

• C12P7/06 Primary

  Ethanol, i.e. non-beverage · CPC title

• C12N1/18

  Baker's yeast; Brewer's yeast · CPC title

• C12R1/865

  Chemistry & Metallurgy · mapped topic

• C12P7/12

  substrate containing sulfite waste liquor or citrus waste · CPC title

• Y02E50/17

  Cross-Sectional Technologies · mapped topic