Light-sensitive ion-passing molecules

Patent metadata

Field	Value
Publication number	US-9604073-B2
Application number	US-201615153299-A
Country	US
Kind code	B2
Filing date	May 12, 2016
Priority date	Mar 17, 2010
Publication date	Mar 28, 2017
Grant date	Mar 28, 2017

Abstract

The invention provides polynucleotides and methods for expressing light-activated proteins in animal cells and altering an action potential of the cells by optical stimulation. The invention also provides animal cells and non-human animals comprising cells expressing the light-activated proteins.

First claim

What is claimed is:

1. A system comprising:
a) a delivery device comprising a polynucleotide that comprises a nucleotide sequence encoding a light-activated polypeptide, wherein the light-activated polypeptide comprises, from N-terminus to C-terminus:
i) a core amino acid sequence that is at least 95% identical to the sequence shown in SEQ ID NO:3, SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:4;
ii) an endoplasmic reticulum (ER) export signal; and
iii) a membrane trafficking signal;

b) a light source; and
c) a control device that controls generation of light by the light source.

2. The system of claim 1 , wherein the control device comprises a control circuit that is arranged to respond to an external signal.

3. The system of claim 1 , wherein the control device comprises a control circuit that comprises one or more of a rectifier circuit, a battery, a pulse timer, and a comparator circuit.

4. The system of claim 1 , wherein the control device comprises a control circuit that comprises an integrated circuit.

5. The system of claim 1 , wherein all or a part of the system is implantable.

6. The system of claim 1 , wherein the polynucleotide is present in a matrix.

7. The system of claim 1 , wherein the polynucleotide is present in a gel.

8. The system of claim 1 , wherein the light source comprises a light-emitting diode.

9. The system of claim 1 , wherein the light source comprises a fiber optic.

10. The system of claim 1 , wherein the ER export signal comprises the amino acid sequence FCEYENEV (SEQ ID NO:12).

11. The system of claim 1 , wherein the membrane trafficking signal comprises the amino acid sequence KSRITSEGEYIPLDQIDINV (SEQ ID NO:11).

12. The system of claim 1 , wherein the core amino acid sequence is at least 95% identical to amino acids 25-265 of SEQ ID NO:2.

13. The system of claim 1 , wherein the core amino acid sequence is at least 95% identical to SEQ ID NO:3.

14. The system of claim 1 , wherein the core amino acid sequence is at least 95% identical to SEQ ID NO:4.

15. The system of claim 1 , wherein the light-activated protein-encoding nucleotide sequence is operably linked to a promoter.

16. The system of claim 15 , wherein the promoter is a CaMKIIa promoter.

17. The system of claim 15 , wherein the promoter is a synapsin I promoter.

18. The system of claim 1 , wherein the polynucleotide is in an expression vector.

19. The system of claim 18 , wherein the expression vector is a viral vector.

20. The system of claim 19 , wherein the viral vector is an adenoassociated virus vector, a retroviral vector, an adenoviral vector, a herpes simplex virus vector, or a lentiviral vector.

Assignees

• Univ Leland Stanford Junior

Inventors

• Deisseroth Karl
• Zhang Feng
• Gradinaru Viviana

Classifications

• A01K2267/0393

  Animal model comprising a reporter system for screening tests · CPC title

• C12N2740/15043

  viral genome or elements thereof as genetic vector · CPC title

• A01K67/0275

  Genetically modified vertebrates, e.g. transgenic · CPC title

• C07K19/00

  Hybrid peptides {, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes} · CPC title

• A01K67/0271 Primary

  Chimeric vertebrates, e.g. comprising exogenous cells · CPC title