Microorganism of the genus Escherichia having enhanced L-tryptophan productivity and a method for producing L-tryptophan using the same

Patent metadata

Field	Value
Publication number	US-9587261-B2
Application number	US-201314371465-A
Country	US
Kind code	B2
Filing date	Jan 10, 2013
Priority date	Jan 10, 2012
Publication date	Mar 7, 2017
Grant date	Mar 7, 2017

Abstract

The present invention relates to microorganisms of Escherichia coli having enhanced L-tryptophan productivity and to a method for producing L-tryptophan using the same. More particularly, the present invention relates to an Escherichia coli variant in which repression and attenuation control of the tryptophan operon is released and accumulation of anthranilate is reduced and thereby enhancing L-tryptophan productivity. The present invention also relates to a method for producing L-tryptophan using the Escherichia coli variant.

First claim

The invention claimed is:

1. A recombinant microorganism of the genus Escherichia having an enhanced L-tryptophan productivity compared to the L-tryptophan productivity of a corresponding wild-type Escherichia microorganism,
wherein the recombinant microorganism has a modification to a chromosomal tryptophan operon, wherein the modification is a deletion of part or all of the nucleotide sequence of SEQ ID NO: 2 in an expression regulatory region comprising the nucleotide sequence of SEQ ID NO: 1,
wherein the recombinant microorganism is transformed with tryptophan operon genes trpD, trpC, trpB, and trpA for increased expression of said tryptophan operon genes as compared to the corresponding wild-type Escherichia microorganism, and wherein the recombinant microorganism does not have increased expression of a trpE gene or increased activity of anthranilate synthase encoded by the trpE gene as compared to the corresponding wild-type Escherichia microorganism.

2. The recombinant microorganism according to claim 1 , wherein the recombinant microorganism has an additional modification to the chromosomal tryptophan operon, wherein the modification is a deletion of part or all of the nucleotide sequence of SEQ ID NO: 3 in the expression regulatory region comprising the nucleotide sequence of SEQ ID NO: 1.

3. The recombinant microorganism according to claim 2 , wherein the trpD gene encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:37, the trpC gene encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:38, the trpB gene encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:39, and the trpA gene encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:40.

4. The recombinant microorganism according to claim 1 , wherein the recombinant microorganism is an Escherichia coli strain.

5. A method for producing L-tryptophan, comprising culturing the recombinant microorganism of claim 1 under conditions suitable for the production of L-tryptophan.

6. The method according to claim 5 , wherein the recombinant microorganism has a further modification to a chromosomal tryptophan operon, wherein the modification is a deletion of part or all of the nucleotide sequence of SEQ ID NO: 3 in the expression regulatory region comprising the nucleotide sequence of SEQ ID NO: 1.

7. The method according to claim 5 , wherein the recombinant microorganism is an Escherichia coli strain.

Assignees

• Cj Cheiljedang Corp

Inventors

• Lee Kwang Ho
• Park Hye Min
• Lee Hyo Hyoung
• Hwang Young Bin
• Lee Seok Myung

Classifications

• C12P13/227 Primary

  Tryptophan · CPC title

• C12N15/71

  Expression systems using regulatory sequences derived from the trp-operon · CPC title

• C07K14/245

  Escherichia (G) · CPC title

• H01L27/3276

  Electricity · mapped topic

• H01L51/5231

  Electricity · mapped topic