Ubiquitin regulatory elements

Patent metadata

Field	Value
Publication number	US-9587243-B2
Application number	US-201414539673-A
Country	US
Kind code	B2
Filing date	Nov 12, 2014
Priority date	Aug 27, 2008
Publication date	Mar 7, 2017
Grant date	Mar 7, 2017

Abstract

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from sorghum . A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

First claim

What is claimed is:

1. A regulatory element that drives transcription in a constitutive manner, wherein the regulatory element comprises a nucleotide sequence selected from the group consisting of:
a) the nucleotide sequence of SEQ ID NO: 1, 2 or 3; and
b) a nucleotide sequence that is at least 90% identical to the nucleotide sequence of SEQ ID NOS: 1 or 2 wherein, the regulatory element is operably linked to a heterologous nucleotide sequence.

2. An expression cassette comprising a regulatory element operably linked to a nucleotide sequence wherein the regulatory element comprises the nucleotide sequence of claim 1 .

3. A plant stably transformed with an expression cassette of claim 2 .

4. The plant of claim 3 , wherein said plant is a monocot.

5. The plant of claim 3 , wherein said monocot is maize, wheat, rice, barley, sorghum or rye.

6. Seed of the plant of claim 3 wherein the seed comprises the expression cassette.

7. A method for selectively expressing a nucleotide sequence in a plant cell, the method comprising:
a) transforming a plant cell with an expression cassette, the expression cassette comprising a regulatory element, wherein the regulatory element comprises a nucleotide sequence selected from the group consisting of:
i) the nucleotide sequence set forth in SEQ ID NO: 1, 2 or 3; and
ii) a nucleotide sequence that is at least 90% identical to the nucleotide sequence of SEQ ID NOS: 1 or 2; wherein the regulatory element drives the expression of a heterologous nucleotide sequence and

b) growing the plant cell to selectively express the nucleotide sequence.

8. The method of claim 7 further comprising regenerating a stably transformed plant from the plant cell; wherein expression of the nucleotide sequence alters the phenotype of a plant.

9. The plant of claim 8 , wherein said plant is a monocot.

10. The plant of claim 8 , wherein said monocot is maize, wheat, rice, barley, sorghum or rye.

11. Seed of the plant of claim 8 wherein the seed comprises the expression cassette.

Assignees

• Pioneer Hi Bred Int
• Pioneer Hi Bred Int

Inventors

• Abbitt Shane

Classifications

• C12N15/8216 Primary

  Methods for controlling, regulating or enhancing expression of transgenes in plant cells · CPC title

• C12N15/823

  Reproductive tissue-specific promoters · CPC title

• C12N15/113

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N15/8223 Primary

  Vegetative tissue-specific promoters · CPC title